DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Joe, Cheol-O | - |
dc.contributor.advisor | 조철오 | - |
dc.contributor.author | Park, Jin-hwi | - |
dc.contributor.author | 박진휘 | - |
dc.date.accessioned | 2011-12-12T07:55:18Z | - |
dc.date.available | 2011-12-12T07:55:18Z | - |
dc.date.issued | 2008 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=295340&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27645 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2008.2, [ viii, 100 p. ] | - |
dc.description.abstract | Biochemical mechanisms by which NF-κB transcriptional activity is modulated by nuclear protein Daxx and SPOP were proposed. Both chromatin immunoprecipitation (ChIP) assay and electrophoretic mobility shift assay (EMSA) have confirmed Daxx-mediated repression of transcriptional competence of NF-κB in HeLa cells. Overexpression of Daxx repressed the expression of NF-κB-regulated genes such as IκBα and IL8. Coimmunoprecipitation assay revealed the existence of intermolecular association between endogenous Daxx and p65 subunit of NF-κB stimulated by TNFα. The sensitization of TNFα-induced apoptosis by Daxx involves the downregulation of NF-κB dependent transcription of antiapoptotic c-FLIP in HeLa cells undergoing TNFα-induced apoptosis. Daxx-mediated repression of NF-κB transactivation is suggested to correlate with the inhibition of p65 acetylation by Daxx. Based on the finding that Daxx binding N-terminal side of p65 includes the major sites of acetylation mediated by p300/CBP, the physical interaction between Daxx and p65 may provide a functional framework for the inhibition of p65 acetylation by p300/CBP and subsequent repression of NF-κB transcriptional activity. Also observed was the inhibition of NF-κB transcriptional capacity by SPOP which interfere with the DNA binding ability of NF-κB to its recognition in the nuclei of cotransfected HeLa cells. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Daxx | - |
dc.subject | SPOP | - |
dc.subject | NF- κB | - |
dc.subject | Transcriptional modulation | - |
dc.subject | Daxx | - |
dc.subject | SPOP | - |
dc.subject | NF- κB | - |
dc.subject | 전사 조절 | - |
dc.subject | Daxx | - |
dc.subject | SPOP | - |
dc.subject | NF- κB | - |
dc.subject | Transcriptional modulation | - |
dc.subject | Daxx | - |
dc.subject | SPOP | - |
dc.subject | NF- κB | - |
dc.subject | 전사 조절 | - |
dc.title | Modulation of NF- κB Transcriptional activity by nuclear protein Daxx and SPOP | - |
dc.title.alternative | 핵 내 단백질인 Daxx와 SPOP에 의한 NF- κB의 전사활성 억제 기작 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 295340/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020045110 | - |
dc.contributor.localauthor | Joe, Cheol-O | - |
dc.contributor.localauthor | 조철오 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.