Modulation of NF- κB Transcriptional activity by nuclear protein Daxx and SPOP = 핵 내 단백질인 Daxx와 SPOP에 의한 NF- κB의 전사활성 억제 기작

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Biochemical mechanisms by which NF-κB transcriptional activity is modulated by nuclear protein Daxx and SPOP were proposed. Both chromatin immunoprecipitation (ChIP) assay and electrophoretic mobility shift assay (EMSA) have confirmed Daxx-mediated repression of transcriptional competence of NF-κB in HeLa cells. Overexpression of Daxx repressed the expression of NF-κB-regulated genes such as IκBα and IL8. Coimmunoprecipitation assay revealed the existence of intermolecular association between endogenous Daxx and p65 subunit of NF-κB stimulated by TNFα. The sensitization of TNFα-induced apoptosis by Daxx involves the downregulation of NF-κB dependent transcription of antiapoptotic c-FLIP in HeLa cells undergoing TNFα-induced apoptosis. Daxx-mediated repression of NF-κB transactivation is suggested to correlate with the inhibition of p65 acetylation by Daxx. Based on the finding that Daxx binding N-terminal side of p65 includes the major sites of acetylation mediated by p300/CBP, the physical interaction between Daxx and p65 may provide a functional framework for the inhibition of p65 acetylation by p300/CBP and subsequent repression of NF-κB transcriptional activity. Also observed was the inhibition of NF-κB transcriptional capacity by SPOP which interfere with the DNA binding ability of NF-κB to its recognition in the nuclei of cotransfected HeLa cells.
Advisors
Joe, Cheol-Oresearcher조철오researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2008
Identifier
295340/325007  / 020045110
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2008.2, [ viii, 100 p. ]

Keywords

Daxx; SPOP; NF- κB; Transcriptional modulation; Daxx; SPOP; NF- κB; 전사 조절; Daxx; SPOP; NF- κB; Transcriptional modulation; Daxx; SPOP; NF- κB; 전사 조절

URI
http://hdl.handle.net/10203/27645
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=295340&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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