Analysis of the H19ICR insulator = H19ICR의 insulator 기능 분석

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Transcriptional insulators are specialized cis-acting elements that protect promoters from inappropriate activation by distal enhancers. The $H19 Imprinting Control Region (ICR)$ functions as a CTCF-dependent, methylation-sensitive transcriptional insulator. Several insertional mutations and knock-out mouse were examined to demonstrate the H19ICR functions as a methylation-regulated maternal-specific insulator in novel chromosomal contexts. To investigate the configuration of cis-acting elements at several insertion sites, Chromosome Conformation Capture (3C) and Chromatin immunoprecipitation (ChIP) assays were used. By comparing maternal and paternal organizations on wild type and mutant chromosomes, mechanisms for ICR/insulator function were identified. The results indicate that promoter and enhancer element invariably associate to form DNA loop domains at transcriptionally active loci. Conversely, active insulators always prevent these promoter-enhancer interactions. Instead the ICR/insulator forms novel loop domains by associating with the blocked promoters and enhancers. The results suggest that these associations are fundamental to insulator function.
Advisors
Chung, Jae-Hoonresearcher정재훈researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2008
Identifier
295338/325007  / 020037415
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2008.2, [ v, 112 p. ]

Keywords

H19ICR; epigenetics; imprinted gene; transcriptional insulator; transcriptional regulation; 후생유전학; 각인 유전자; 전사 절연체; 전자 조절; H19ICR; epigenetics; imprinted gene; transcriptional insulator; transcriptional regulation; 후생유전학; 각인 유전자; 전사 절연체; 전자 조절

URI
http://hdl.handle.net/10203/27643
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=295338&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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