DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chung, Jong-Kyeong | - |
dc.contributor.advisor | 정종경 | - |
dc.contributor.author | Kim, Myung-Jin | - |
dc.contributor.author | 김명진 | - |
dc.date.accessioned | 2011-12-12T07:54:51Z | - |
dc.date.available | 2011-12-12T07:54:51Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=258104&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27615 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2006.8, [ xiii, 187 p. ] | - |
dc.description.abstract | The Ras/extracellular signal-regulated kinase (ERK) and the NF-kB signaling pathways are critically involved in diverse biological processes, including mitogenesis, cellular differentiation, and immune reactions. 1) Using gain-of-function and null mutants of RSK, its physiological role was characterized in Drosophila. Surprisingly, RSK-null mutants were viable, but exhibited developmental abnormalities related to an enhanced ERK-dependent cellular differentiation such as ectopic photoreceptor- and vein-cell formation. Conversely, overexpression of RSK dramatically suppressed the ERK-dependent differentiation, which was further augmented by mutations in the Ras/ERK pathway. Consistent with these physiological phenotypes, RSK negatively regulated ERK-mediated developmental processes and gene expressions by blocking the nuclear localization of ERK in a kinase activity-independent manner. Moreover, I further demonstrated that the RSK-dependent inhibition of ERK nuclear migration is mediated by the physical association between ERK and RSK. 2) Mitogen-activated protein kinase (MAPK) phosphatase 3 (MKP-3) is a well-known negative regulator in the Ras/ERK signaling pathway. Here, I demonstrated the physiological function of endogenous MKP-3 through the loss-of-function and null mutant-based studies. Although the DMKP-3 hypomorphic mutants well survived into adults with extra photoreceptor- and vein-cells, DMKP-3 null mutants showed embryonic lethality and severe oogenesis defects, demonstrating that MKP-3 is essential for the normal development in Drosophila. All of these phenotypes were highly similar to those of the gain-of-function mutants of ERK, and the functional interaction between MKP-3 and the Ras/ERK pathway was further confirmed by genetic interaction assays. 3) To find novel suppressors of the NF-kB signaling pathway, I performed a genetic screening for Drosophila mutants with hyper-activated immune responses, and isolated a loss-of-function mutant of caspa... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | cell differentiation | - |
dc.subject | immunity | - |
dc.subject | 면역계 | - |
dc.subject | 세포분화 | - |
dc.title | Regulation of ERK and $NF-\kappa B$ pathways in drosophila development | - |
dc.title.alternative | ERK와 $NF-\kappa B$ 신호전달계의 조절기작 규명 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 258104/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020035031 | - |
dc.contributor.localauthor | Chung, Jong-Kyeong | - |
dc.contributor.localauthor | 정종경 | - |
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