Production of lycopene and β-Carotene from metabolically engineered escherichia coli = 대사공학적으로 개량된 대장균으로부터 라이코펜과 베타카로텐의 생산에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 283
  • Download : 0
ABSTRACT We generated recombinant lycopene and β-carotene utilizing metabolically engineered Escherichia coli DH5α. For this purpose, we constructed synthetic operons regulated by the araBAD promoter. Vectors harboring these operons exhibited diverse expression patterns dependant upon plasmid copy number. Carotenoid production from these recombinant E. coli was either constitutive or inducible depending upon plasmid copy number. Furthermore, the yields of both lycopene and β-carotene in E. coli cultures harboring high-copy vectors (pTB-EIB and pTB-EIBY) varied depending upon inocula state. This inocula dependence, solely associated with use of high copy vectors, was overcome through using low-copy vectors (pTB-EIBrop and pTB-EIBYrop) for recombinant carotenoid production. We performed fermentation process from laboratory-scale to pilot-scale. Various cultivation conditions, including temperature, medium composition, and induction time, were investigated and determined for high-level production of lycopene and β-carotene. Interestingly, lycopene and β-carotene were greatly increased at low-temperature condition (25 ℃). Through fed-batch fermentation in 300 L-bioreactor, maximal lycopene production reached up to 99 $mg/l^{-1}$ with 2.3 $mg/g^{-1}$ DCW. In addition, using this same technique, β-carotene production reached up to 110 $mg/ l^{-1}$ with 2.2 $mg/g^{-1}$ DCW. Although carotenoid content per cell is relatively low, our results showed that the high-level production of carotenoids could be achieved by optimizing culture conditions at various fermentation scales. Previous sequence analyses of the lycopene cyclase gene (crtY) revealed the protein product was translated from the ATG start codon in Escherichia coli. We found, however, this enzyme could be also obtained without ATG start codon. The research using crtY mutants revealed the GTG (Val) sequence located in-frame 24 bp downstream of the ATG was a potential start codon. This result suggests t...
Advisors
Kim, Jung-Hoeresearcher김정회researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2006
Identifier
258103/325007  / 020025815
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2006.8, [ vii, 112 p. ]

Keywords

Lycopene; β-Carotene; Metabolic engineering; 대사공학; 라이코펜; 베타카로텐

URI
http://hdl.handle.net/10203/27614
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=258103&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0