Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

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dc.contributor.authorShemesh, Or A.ko
dc.contributor.authorLinghu, Changyangko
dc.contributor.authorPiatkevich, Kiryl D.ko
dc.contributor.authorGoodwin, Danielko
dc.contributor.authorCeliker, Orhan Tuncko
dc.contributor.authorGritton, Howard J.ko
dc.contributor.authorRomano, Michael F.ko
dc.contributor.authorGao, Ruixuanko
dc.contributor.authorYu, Chih-Chieh (Jay)ko
dc.contributor.authorTseng, Hua-Anko
dc.contributor.authorBensussen, Sethko
dc.contributor.authorNarayan, Sujathako
dc.contributor.authorYang, Chao-Tsungko
dc.contributor.authorFreifeld, Limorko
dc.contributor.authorSiciliano, Cody A.ko
dc.contributor.authorGupta, Ishanko
dc.contributor.authorWang, Joyceko
dc.contributor.authorPak, Nikitako
dc.contributor.authorYoon, Young-Gyuko
dc.contributor.authorUllmann, Jeremy F. P.ko
dc.contributor.authorGuner-Ataman, Burcuko
dc.contributor.authorNoamany, Habibako
dc.contributor.authorSheinkopf, Zoe R.ko
dc.contributor.authorPark, Won Minko
dc.contributor.authorAsano, Shohko
dc.contributor.authorKeating, Amy E.ko
dc.contributor.authorTrimmer, James S.ko
dc.contributor.authorReimer, Jacobko
dc.contributor.authorTolias, Andreas S.ko
dc.contributor.authorBear, Mark F.ko
dc.contributor.authorTye, Kay M.ko
dc.contributor.authorHan, Xueko
dc.contributor.authorAhrens, Misha B.ko
dc.contributor.authorBoyden, Edward S.ko
dc.date.accessioned2020-08-24T09:55:04Z-
dc.date.available2020-08-24T09:55:04Z-
dc.date.created2020-06-27-
dc.date.created2020-06-27-
dc.date.issued2020-08-
dc.identifier.citationNEURON, v.107, no.3, pp.470 - +-
dc.identifier.issn0896-6273-
dc.identifier.urihttp://hdl.handle.net/10203/275931-
dc.description.abstractMethods for one-photon fluorescent imaging of calciumdynamics can capture the activity of hundreds of neurons across large fields of view at a low equipment complexity and cost. In contrast to two-photon methods, however, one-photon methods suffer from higher levels of crosstalk from neuropil, resulting in a decreased signal-to-noise ratio and artifactual correlations of neural activity. We address this problem by engineering cell-body-targeted variants of the fluorescent calcium indicators GCaMP6f and GCaMP7f. We screened fusions of GCaMP to natural, as well as artificial, peptides and identified fusions that localized GCaMP to within 50 mmof the cell body of neurons in mice and larval zebrafish. One-photon imaging of soma-targetedGCaMPin dense neural circuits reported fewer artifactual spikes from neuropil, an increased signal-to-noise ratio, and decreased artifactual correlation across neurons. Thus, soma-targeting of fluorescent calcium indicators facilitates usage of simple, powerful, one-photon methods for imaging neural calcium dynamics.-
dc.languageEnglish-
dc.publisherCELL PRESS-
dc.titlePrecision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator-
dc.typeArticle-
dc.identifier.wosid000556135600001-
dc.identifier.scopusid2-s2.0-85087664780-
dc.type.rimsART-
dc.citation.volume107-
dc.citation.issue3-
dc.citation.beginningpage470-
dc.citation.endingpage+-
dc.citation.publicationnameNEURON-
dc.identifier.doi10.1016/j.neuron.2020.05.029-
dc.contributor.localauthorYoon, Young-Gyu-
dc.contributor.nonIdAuthorShemesh, Or A.-
dc.contributor.nonIdAuthorLinghu, Changyang-
dc.contributor.nonIdAuthorPiatkevich, Kiryl D.-
dc.contributor.nonIdAuthorGoodwin, Daniel-
dc.contributor.nonIdAuthorCeliker, Orhan Tunc-
dc.contributor.nonIdAuthorGritton, Howard J.-
dc.contributor.nonIdAuthorRomano, Michael F.-
dc.contributor.nonIdAuthorGao, Ruixuan-
dc.contributor.nonIdAuthorYu, Chih-Chieh (Jay)-
dc.contributor.nonIdAuthorTseng, Hua-An-
dc.contributor.nonIdAuthorBensussen, Seth-
dc.contributor.nonIdAuthorNarayan, Sujatha-
dc.contributor.nonIdAuthorYang, Chao-Tsung-
dc.contributor.nonIdAuthorFreifeld, Limor-
dc.contributor.nonIdAuthorSiciliano, Cody A.-
dc.contributor.nonIdAuthorGupta, Ishan-
dc.contributor.nonIdAuthorWang, Joyce-
dc.contributor.nonIdAuthorPak, Nikita-
dc.contributor.nonIdAuthorUllmann, Jeremy F. P.-
dc.contributor.nonIdAuthorGuner-Ataman, Burcu-
dc.contributor.nonIdAuthorNoamany, Habiba-
dc.contributor.nonIdAuthorSheinkopf, Zoe R.-
dc.contributor.nonIdAuthorPark, Won Min-
dc.contributor.nonIdAuthorAsano, Shoh-
dc.contributor.nonIdAuthorKeating, Amy E.-
dc.contributor.nonIdAuthorTrimmer, James S.-
dc.contributor.nonIdAuthorReimer, Jacob-
dc.contributor.nonIdAuthorTolias, Andreas S.-
dc.contributor.nonIdAuthorBear, Mark F.-
dc.contributor.nonIdAuthorTye, Kay M.-
dc.contributor.nonIdAuthorHan, Xue-
dc.contributor.nonIdAuthorAhrens, Misha B.-
dc.contributor.nonIdAuthorBoyden, Edward S.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusBRAIN ACTIVITY-
dc.subject.keywordPlusK+ CHANNEL-
dc.subject.keywordPlusDYNAMICS-
dc.subject.keywordPlusRESOLUTION-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusTRAFFICKING-
dc.subject.keywordPlusSIGNALS-
dc.subject.keywordPlusBINDING-
dc.subject.keywordPlusCORTEX-
dc.subject.keywordPlusSPEED-
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