Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging

Cited 1 time in webofscience Cited 0 time in scopus
  • Hit : 83
  • Download : 9
3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45 degrees reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures.
Publisher
MDPI
Issue Date
2020-06
Language
English
Article Type
Article
Citation

MICROMACHINES, v.11, no.6

ISSN
2072-666X
DOI
10.3390/mi11060582
URI
http://hdl.handle.net/10203/275817
Appears in Collection
NT-Journal Papers(저널논문)
Files in This Item
000551587400001.pdf(4.61 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 1 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0