Minimization of the escherichia coli genome using a Tn5-coupled Cre/loxP excision system = 트랜스포존 Tn5와 Cre/loxP을 이용한 대장균 유전체의 축소 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 275
  • Download : 0
Many genome sequences of useful industrial microorganisms have been completely sequenced. Based on the results of these genome sequences, functional analyses of microbial genomes have been carried out using comparative genomics and bioinformatics to define functions of new genes and metabolic pathways. These genomic analyses have also been performed in the Escherichia coli which is an important experimental and industrial organism and 620 essential genes and 3,126 nonessential genes have been identified. Of the nonessential genes, E. coli possesses transposable elements, phages, cryptic prophages, motility related genes, horizontally transferred genes, pseudogenes, gene remnants, and damaged operons that would not be needed in the strains designed for biotechnology purposes. These nonessential genes express many unnecessary gene products that represent potential contaminants and metabolic wastes entailed in producing unwanted products that could drive up the cost of product purification and are extra burdens on cell growth. In addition, the genetic instability of the microorganism owing to transposable elements is certainly a problem for producing useful biomaterials and rational strain improvement. Therefore, deletion of these nonessential genes allows us to improve the microorganism for biotechnology purposes. However, conventional methods for deleting many nonessential genes scattered along the E. coli genome have limitations that these methods require either the creation of targeting vectors or complex PCR experiments per each deletion. The need for numerous deletion experiments led us to develop a rapid and efficient deletion procedure. Here, we have developed a powerful genome-engineering tool to delete the nonessential genes of E. coll. We have made two large pools of independent transposon mutants in E. coli using modified Tn5 transposons with a loxP site and two different selection markers and precisely mapped the chromosomal location of 1,000 of these...
Advisors
Kim, Sun-Changresearcher김선창researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
237520/325007  / 000995237
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2004.2, [ viii, 86 p. ]

Keywords

ESCHERICHIA COLI; MINIMIZATION OF GENOME; TRANSPOSON; 트랜스포존; 대장균; 유전체 축소

URI
http://hdl.handle.net/10203/27571
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237520&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0