Modification of sugar composition in strawberry fruit by antisense suppression of an ADP-glucose pyrophosphorylase = ADP-Glucose Pyrophosphorylase 유전자의 발현 억제에 의한 딸기의 당 조성 변화에 대한 특성

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 299
  • Download : 0
Cultivated strawberry (Fragaria x ananassa Duch.) is an economically very important fruit crop in the world. The aim of this study was to investigate the changes of soluble sugar composition in strawberry fruit. We isolated full cDNA encoding an AGPase small subunit (FagpS) from strawberry cv. Niyobou. The nucleotide length of FagpS cDNA clone was 1826bp. which the cDNA has an open reading frame deriving 57kDa. To modulate the soluble sugar contents of fruits, we obtained transgenic plants that incorporate an antisense orientation of FagpS in strawberry cv. Anther under the control of the fruit-dominant ascorbate peroxidase (APX) promoter. Several independent transgenic and regenerated plants were obtained and propagated in the greenhouse for agronomical tract analysis. Most transgenic fruits did not show significant differences in weight, and hardness when compared with control fruits of strawberry. While starch contents in fruit of transgenic lines were decreased about 27% to 69%, total soluble sugar contents were increased about 16% to 37% in transgenic plants. These results show HPLC analysis of sugar composition at four stages of fruit development, the increase of sugar contents of fruits in transgenic lines compared with wild plants particularly detected in the transition from the turning to the red stage. This results were confirmed by northern analysis which showed the steady-state levels of AGPase mRNA drastically reduced in red stage of fruits in all the transgenic plants. Therefore, these results supported that AGPase expression in the transgenic lines was controlled by APX promoter. The analysis of AGPase transcript levels in other tissues transgenic plants showed that AGPase mRNA expression is similar to control plants. Starch staining of leaf and pollen by I2-KI also show the similar data compared with wild plants. Therefore, our results indicated that AGPase gene is a candidate for changes of sugar composition in strawberry fruit.
Advisors
Chung, Won-Ilresearcher정원일researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
237528/325007  / 000985146
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2004.2, [ xi, 86 p. ]

Keywords

SUGAR/STARCH; METABOLIC ENGINEERING; AGPASE; STRAWBERRY; ANTISENSE; 발현억제; 안티센스; 당/녹말대사; 대사유전자제어; 딸기

URI
http://hdl.handle.net/10203/27562
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237528&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0