DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoo, Ook-Joon | - |
dc.contributor.advisor | 유욱준 | - |
dc.contributor.author | Yoo, Seung-Hee | - |
dc.contributor.author | 유승희 | - |
dc.date.accessioned | 2011-12-12T07:54:00Z | - |
dc.date.available | 2011-12-12T07:54:00Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=237525&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27559 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2004.2, [ viii, 102 p. ] | - |
dc.description.abstract | Many physiological and behavioral systems are controlled by an internal self-sustained molecular oscillating mechanism with a periodicity of approximately 24 hours, known as the biological clock. This self-sustaining clock exists in animals, plants and even in bacteria and adjusted by the alternating of the day and night of the earth``s cycle. This biological rhythms that persist under constant conditions and has a periodicity of - 1 day is called "circadian"(cirda "around"; dian, "day"). This clock system have the cell-autonomous, genetically-based biological propertues with which to respond appropriately to daily changes in environmental stimuli, the most important of which is the light-dark cycle. To understand most remarkable feature of clock gene; circadian oscillation, we made mPer2 real time reporting system by making mPer2:luciferase functional knock-in mice and identified a noncanonical E-box enhancer sequence in the 5``-UTR of the mPer2 gene and used this sequence to drive the circadian expression of luciferase in transgenic mice. mPer2:luciferase functional knock-in mice showed persistent oscillation of bioluminescence with ca. 24 hours of period. Behavior analysis of mPer2:luciferase mouse showed normal behavior compare to same littermate wild type mouse. We demonstrate that the E-box enhancer: luciferase transgenic line and the mPer2: Luciferase knock-in line allow us to monitor in real-time the transcriptional and translational profiles, respectively, of the mPer2 gene in explanted SCN and peripheral tissues. SCN and peripheral tissues showed robust and self-sustained sustained circadian rhythms of mPer2 expression with only slight damping in static explant cultures for up to 20 days. Furthermore, in SCN-lesioned knock-in animals, a persistent circadian oscillation in bioluminescence in peripheral tissues was obserbed, yet from tissue to tissue within each animal and among animals there is a loss of phase coordination. Contrary to previous studies... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | MPER2 REAL-TIME REPORTING | - |
dc.subject | 실시간 리포팅 | - |
dc.subject | 쥐 피어리어드 2 유전자 | - |
dc.title | Circadian systems biology revealed by mPer2 real-time reporting | - |
dc.title.alternative | 쥐 피어리어드 2 유전자 발현 실시간 리포팅에 의해 밝혀진 생체시계 시스템 : 지속적, 조직 특이적인 생체시계 진동자 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 237525/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 000975226 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.localauthor | 유욱준 | - |
dc.title.subtitle | persistent, tissue-specific circadian oscillators | - |
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