Nitric oxide-mediated anti-proliferative effects of ginseng polysaccharide in mice = NO를 매개로 한 세포증식 억제에 미치는 인삼 다당체의 영향 연구

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Acidic polysaccharide from the ethanol-insoluble and water-soluble fraction of Korean red ginseng was isolated and its immunomodulating activities were investigated. Red ginseng acidic polysaccharide (RGAP), administered intraperitoneally for seven days, induced a high output nitric oxide synthase (NOS2) in vivo. In the presence of interferon y , RGAP induced NOS2 by newly synthesized mRNA in vitro. After the multiple injections of RGAP, spleen cells were proliferated spontaneously in a dose dependent manner, while the concanavalin A (Con A)-induced spleen cell proliferation was inhibited. An addition of NG-monomethyl-L-arginine (NMMA) resumed the Con A-induced spleen cell proliferation. The in vivo pretreatment of aminoguanidine, a specific NOS2 inhibitor, alleviated the RGAP-induced suppressions of antibody plaque-forming cell response to sheep red blood cells as well as cytotoxic T lymphocyte response against P815 tumor cells. RGAP-administration also enhanced the macrophage-mediated tumor killing. In the coculture system of P815 cells with macrophages, proliferation of P815 cells was severely suppressed by the RGAP-activated macrophages. NMMA addition alleviated the suppressive proliferation of P815 cells at the same time as blocked NO accumulation. These results implicate a link between NO production and suppression of the proliferative response. The present study proposes that NO is a pivotal mediator of immunomodulating activities of RGAP.
Advisors
Joe, Cheol-O.researcher조철오researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
2002
Identifier
174544/325007 / 000985126
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생물과학과, 2002.2, [ viii, 109 p. ]

Keywords

immunosuppression; macrophage; nitric oxide; ginseng polysaccharide; tumor killing; 항암작용; 세포증식; 면역억제; 대식세포; 인삼 다당체

URI
http://hdl.handle.net/10203/27515
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=174544&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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