Transcriptional regulation of the Wilson disease gene(ATP7B) = 윌슨씨 유전자 (ATP7B) 의 전사조절 연구

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Wilson disease (WD), an autosomal recessive inherited disorder of copper transport, is marked by impaired biliary excretion and incorporation of copper into ceruloplasmin. Clinical features of the WD are characterized by hepatic cirrhosis and neuronal degeneration, which result from toxic levels of copper that accumulate in the liver and brain, respectively. To investigate the molecular mechanisms regulating expression of the WD gene, we isolated, sequenced, and characterized ~1.3 kb of the 5``-flanking region of the WD gene from the human genomic library. The ~1.3 kb of the WD sequence directed high level of luciferase activity in HepG2 cells. Interestingly, sequence analysis showed that the 5``-flanking region contained four metal response elements (MREs)-two of four MREs are in reverse orientation- and six MRE-like sequences (MLSs), usually found in the metallothionein gene. It also contained a number of putative regulatory elements such as Sp1, AP-1, AP-2, and E-box, but lacked TATA box near transcription start site. The transcription start site was located at 335 base pairs upstream from the translation initiation site. Further successive 5``-deletion analyses suggested the important role of the 159-base pair region from -811 to -653, which includes MLS2 (-802 to -796) and MLS3 (-785 to -779), as a positive regulatory element during the expression of the WD gene. A negative element was also identified at region -1038 to -812. A protein-MLS complex was identified through electrophoretic mobility shift and competition assay using MLS2/MLS3 and HepG2 cell nuclear proteins. Among the four MREs, MREa plays the most important role in the transcriptional activation of the WD promoter. Electrophoretic mobility shift assays (EMSAs) using synthetic MREa and an oligonucleotide containing the binding site for transcription factor Sp1 revealed the presence of nuclear factors that bind specifically to MREa. Two MREa-binding proteins of 70- and 82-kilodaltons were pur...
Yoo, Ook-Joonresearcher유욱준researcher
한국과학기술원 : 생물과학과,
Issue Date
169615/325007 / 000975821

학위논문(박사) - 한국과학기술원 : 생물과학과, 2001.8, [ ix, 93 p. ]


MRE(metal response element); promoter; Wilson disease; Ku protein; 스크리닝; 전사조절부위; 윌슨씨 병; RNAi

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