Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex

Cited 2 time in webofscience Cited 0 time in scopus
  • Hit : 109
  • Download : 53
DC FieldValueLanguage
dc.contributor.authorKim, Hyunminko
dc.contributor.authorKubori, Tomokoko
dc.contributor.authorYamazaki, Koheiko
dc.contributor.authorKwak, Mi-Jeongko
dc.contributor.authorPark, Suk-Youlko
dc.contributor.authorNagai, Hirokiko
dc.contributor.authorVogel, Joseph P.ko
dc.contributor.authorOh, Byung-Hako
dc.date.accessioned2020-07-01T08:20:16Z-
dc.date.available2020-07-01T08:20:16Z-
dc.date.created2020-06-26-
dc.date.issued2020-05-
dc.identifier.citationNATURE COMMUNICATIONS, v.11, no.1-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10203/275081-
dc.description.abstractThe Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) is extremely versatile, translocating similar to 300 effector proteins into host cells. This specialized secretion system employs the Dot/Icm type IVB coupling protein (T4CP) complex, which includes IcmS, IcmW and LvgA, that are known to selectively assist the export of a subclass of effectors. Herein, the crystal structure of a four-subunit T4CP subcomplex bound to the effector protein VpdB reveals an interaction between LvgA and a linear motif in the C-terminus of VpdB. The same binding interface of LvgA also interacts with the C-terminal region of three additional effectors, SidH, SetA and PieA. Mutational analyses identified a FxxxLxxxK binding motif that is shared by VpdB and SidH, but not by SetA and PieA, showing that LvgA recognizes more than one type of binding motif. Together, this work provides a structural basis for how the Dot/Icm T4CP complex recognizes effectors, and highlights the multiple substrate-binding specificities of its adaptor subunit. The Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) translocates effector proteins into host cells, and the recognition of these effectors is mediated by the Dot/Icm type IV coupling protein (T4CP) complex. Here, the authors present the crystal structure of a four-subunit containing T4CP subcomplex bound to the effector protein VpdB, and identify a FxxxLxxxK binding motif that is present in a subset of the effectors and which is recognized by the T4CP adaptor subunit LvgA.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleStructural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex-
dc.typeArticle-
dc.identifier.wosid000538809900013-
dc.identifier.scopusid2-s2.0-85085278895-
dc.type.rimsART-
dc.citation.volume11-
dc.citation.issue1-
dc.citation.publicationnameNATURE COMMUNICATIONS-
dc.identifier.doi10.1038/s41467-020-16397-0-
dc.contributor.localauthorOh, Byung-Ha-
dc.contributor.nonIdAuthorKubori, Tomoko-
dc.contributor.nonIdAuthorYamazaki, Kohei-
dc.contributor.nonIdAuthorPark, Suk-Youl-
dc.contributor.nonIdAuthorNagai, Hiroki-
dc.contributor.nonIdAuthorVogel, Joseph P.-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusLEGIONELLA-PNEUMOPHILA-
dc.subject.keywordPlusINTRACELLULAR MULTIPLICATION-
dc.subject.keywordPlusVIRULENCE SYSTEM-
dc.subject.keywordPlusSECRETION SYSTEM-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusLOCUS-
dc.subject.keywordPlusTRANSLOCATION-
dc.subject.keywordPlusFAMILY-
dc.subject.keywordPlusGENES-
dc.subject.keywordPlusTRAG-
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 2 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0