High-level production of 3-hydroxypropionic acid from glycerol as a sole carbon source using metabolically engineered Escherichia coli

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As climate change is an important environmental issue, the conventional petrochemical-based processes to produce valuable chemicals are being shifted toward eco-friendly biological-based processes. In this study, 3-hydroxypropionic acid (3-HP), an industrially important three carbon (C3) chemical, was overproduced by metabolically engineeredEscherichia coliusing glycerol as a sole carbon source. As the first step to construct a glycerol-dependent 3-HP biosynthetic pathway, thedhaB1234andgdrABgenes fromKlebsiella pneumoniaeencoding glycerol dehydratase and glycerol reactivase, respectively, were introduced intoE. colito convert glycerol into 3-hydroxypropionaldehyde (3-HPA). In addition, theydcWgene fromK. pneumoniaeencoding gamma-aminobutyraldehyde dehydrogenase, among five aldehyde dehydrogenases examined, was selected to further convert 3-HPA to 3-HP. Increasing the expression level of theydcWgene enhanced 3-HP production titer and reduced 1,3-propanediol production. To enhance 3-HP production, fed-batch fermentation conditions were optimized by controlling dissolved oxygen (DO) level and employing different feeding strategies including intermittent feeding, pH-stat feeding, and continuous feeding strategies. Fed-batch culture of the final engineeredE. colistrain with DO control and continuous feeding strategy produced 76.2 g/L of 3-HP with the yield and productivity of 0.457 g/g glycerol and 1.89 g center dot L-1 center dot h(-1), respectively. To the best of our knowledge, this is the highest 3-HP productivity achieved by any microorganism reported to date.
Publisher
WILEY
Issue Date
2020-07
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.117, no.7, pp.2139 - 2152

ISSN
0006-3592
DOI
10.1002/bit.27344
URI
http://hdl.handle.net/10203/275076
Appears in Collection
CBE-Journal Papers(저널논문)
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