(The) biochemical properties of hpatitis B Virus(HBV) preS2 region and its role inthe infection mechanism of HBV = Hepatitis B Virus의 표면항원 부위중 preS2 부위의 생화학적 특징및 이의 HBV 감염기작에서의 역할에 관한 연구

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Hepatitis B virus(HBV) has been recognized to be the cause of hepatitis B and numerous studies about its pathogenesis and viral characteristics have been accomplished. However, currently there is no appropriate in vitro system for the study of HBV infection. Only some cell lines, such as HepG2, can be used for the viral replication when transiently or stably transfected with HBV DNA. So, in contrast to well-documented assembly and export of newly synthesized viral particles from the host cell, almost nothing is known about the early infection mechanism. Only the hepatocyte attachment site on the viral surface protein was identified as preS1(21-47) region. However, not yet the receptor for HBV has been isolated. Since the Hepatitis B viron is known to be internalized by receptor-mediated endocytosis via their envelope proteins, it is thinkable that these proteins also play a role in the intracellular transport of the virus. So, to evaluate whether the surface proteins, especially preS region, is involved in the early infection process containing initial attachment and intracellular transport of HBV genome into the host nucleus, the functions of preS region were analyzed. First, the interaction between preS region and polymerized human serum albumin (pHSA) was studied by competitive binding inhibition assay and the structural features of preS2 region contributed to the binding of pHSA was analyzed. And its importance in viral infection and vaccination would be discussed. Second, to investigate another role of preS region in the infection mechanism, intracellular distribution of exogenous pre S protein was visualized by confocal microscopy. The preS proteins were specifically accumulated into the nucleus and this process is energy- and mediator independent. It seems to be because of the permeabilization effect of preS(120-174) sequence on the lipid bilayer. For the more characterization of permeabilization effect of preS2 region on the lipid bilayer, the inter...
Yoo, Ook-JoonresearcherKim, Kil-Lyongresearcher유욱준researcher김길룡researcher
한국과학기술원 : 생물과학과,
Issue Date
166348/325007 / 000935350

학위논문(박사) - 한국과학기술원 : 생물과학과, 2001.2, [ ix, 93 p. ]


Biochemical properties of Hpatitis B Virus(HBV) preS2 region; preS2 부위의 생화학적 특징

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