DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Sung-Taik | - |
dc.contributor.advisor | 이성택 | - |
dc.contributor.author | Cho, Young-Gyun | - |
dc.contributor.author | 조영균 | - |
dc.date.accessioned | 2011-12-12T07:53:00Z | - |
dc.date.available | 2011-12-12T07:53:00Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165686&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27494 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생물과학과, 2001.2, [ viii, 130 p. ] | - |
dc.description.abstract | Nitrophenol-degrading bacteria were isolated from industrial wastewater by selective enrichment cultures. On the basis of their chemotaxonomic and phylogenetic characteristics, p-nitrophenol-degrading strain NSP41 was identified as a new species of the genus Nocardioides (Nocardioides nitrophenolicus $NSP41^T)$ and 2,4-dinitrophenol-degrading strain DNP505 was identified as a new species of the genus Rhodococcus (Rhodococcus koreensis $DNP505^T)$. The effect of phenol supplementation on the degradation of p-nitrophenol by Nocardioides sp. NSP41 was investigated. Strain NSP41 was able to degrade p-nitrophenol and phenol simultaneously. In the same concentration (molar base) of p-nitrophenol and phenol, both compounds were degraded simultaneously at the same rate without accumulation of any other intermediates. In low phenol concentration, phenol was completely mineralized before p-nitrophenol degradation. After exhaustion of phenol, p-nitrophenol was partially transformed to 4-nitrocatechol. Concerning high phenol concentration, p-nitrophenol was degraded faster and completely exhausted before phenol degradation. Kinetically, the addition of phenol enhanced the apparent p-nitrophenol degradation rate, which may be due to the increased cell mass by the assimilation of phenol. The pathway for simultaneous degradation of p-nitrophenol and phenol was investigated in Nocardioides sp. NSP41. Strain NSP41 degraded p-nitrophenol via a hydroquinone pathway, while degraded phenol through a catechol pathway. Resting cells and enzyme extracts from cells grown on p-nitrophenol, phenol, and the mixture of p-nitrophenol and phenol indicated that there are two alternative pathways for the metabolism of p-nitrophenol and phenol in strain NSP41 and two pathways were expressed depending on the substrate used. During the simultaneous degradation of p-nitrophenol and phenol, both hydroquinone pathway and catechol pathway were induced and expressed simultaneously. In order to eluc... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Nocardioides sp. NSP41 | - |
dc.subject | biodegradation | - |
dc.subject | nitrophenol | - |
dc.subject | Rhodococcus sp. DNP505 | - |
dc.subject | 로도코코스 | - |
dc.subject | 노카르디오이데스 | - |
dc.subject | 생물학적 분해 | - |
dc.subject | 니트로페놀 | - |
dc.title | Degradation of nitrophenols by nocardioides sp. NSP41 and rhodococcus sp. DNP505 | - |
dc.title.alternative | Nocardioides sp. NSP41과 rhodococcus sp. DNP505를 이용한 니트로페놀의 생물학적 분해 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 165686/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000965383 | - |
dc.contributor.localauthor | Lee, Sung-Taik | - |
dc.contributor.localauthor | 이성택 | - |
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