DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chung, An-Sik | - |
dc.contributor.advisor | 정안식 | - |
dc.contributor.author | Hong, Sun-Taek | - |
dc.contributor.author | 홍선택 | - |
dc.date.accessioned | 2011-12-12T07:52:58Z | - |
dc.date.available | 2011-12-12T07:52:58Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165633&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27492 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생물과학과, 2001.2, [ x, 112 p. ] | - |
dc.description.abstract | The carboxy-terminus of the gag gene of Mason-Pfizer monkey virus (M-PMV), the prototype immunosuppressive primate type D retrovirus, encodes a 36-amino acid, proline-rich protein domain that, in the mature virion, becomes the p4 capsid protein. The p4 domain has no known role in M-PMV replication. We found that two mutants with premature termination codons that remove half or all of the p4 domain produced lower levels of stable Gag protein and of self-assembled capsids. Interestingly, yeast two-hybrid screening revealed that p4 specifically interacted with TCP-1γ, a subunit of the chaperonin TRiC (TCP-1 ring complex). TRiC is a cytosolic chaperonin that is known to be involved in both folding and subunit assembly of a variety of cellular proteins. TCP-1γ also associated with high specificity with p6, a similar proline-rich domain of human immunodeficiency virus type 1 (HIV-1). Moreover, in cells, Gag polyprotein associated with the TRiC chaperonin complex and this association depended on ATP hydrolysis. In the p4 truncation mutants, the Gag-TRiC association was greatly reduced. These results strongly suggest that the cytosolic chaperonin TRiC is involved in Gag folding and/or capsid assembly. We propose that TRiC associates transiently with nascent M-PMV Gag molecules to assist in their folding. Consequently, properly folded Gag molecules carry out the intermolecular interactions involved in self-assembly of the immature capsid. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 리트로 바이러스 | - |
dc.subject | 샤페론 | - |
dc.subject | Chaperonin | - |
dc.subject | 구조 | - |
dc.subject | Folding | - |
dc.subject | Assembly | - |
dc.title | Studies on the host cell factor functioning in the folding and capsid assembly of gag polyprotein of M-PMV | - |
dc.title.alternative | M-PMV Gag 중합단백질의 구조, 캡시드 형성에 작용하는 숙주세포 인자에 관한 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 165633/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000975426 | - |
dc.contributor.localauthor | Chung, An-Sik | - |
dc.contributor.localauthor | 정안식 | - |
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