DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Younggeun | ko |
dc.contributor.author | Park, Juhwan | ko |
dc.contributor.author | Park, Je-Kyun | ko |
dc.date.accessioned | 2020-06-10T06:20:06Z | - |
dc.date.available | 2020-06-10T06:20:06Z | - |
dc.date.created | 2020-06-08 | - |
dc.date.created | 2020-06-08 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | SENSORS, v.20, no.8 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | http://hdl.handle.net/10203/274590 | - |
dc.description.abstract | Although immunomagnetic separation is a useful sample pretreatment method that can be used to separate target pathogens from a raw sample, it is challenging to remove unbound free magnetic nanoparticles (MNPs) for colorimetric detection of target pathogens. Here, size-based filtration was exploited for the rapid on-site detection of pathogens separated by immunomagnetic separation in order to remove unbound free MNPs using a finger-powered microfluidic device. A membrane filter and an absorbent pad were integrated into the device and a mixture of unbound free MNPs and MNP-bound Escherichia coli (E. coli) O157:H7 was dispensed over the membrane filter by pressing and releasing the pressure chamber. A colorimetric signal was generated by MNP-bound E. coli O157:H7 while unbound free MNPs were washed out by the absorbent. Furthermore, the colorimetric signals can be amplified using a gold enhancer solution when gold-coated MNPs were used instead of MNPs. As a result, 10(2) CFU/mL E. coli O157:H7 could be detected by the enhanced colorimetric signal on a proposed device. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | Colorimetric Detection of Escherichia coli O157:H7 with Signal Enhancement Using Size-Based Filtration on a Finger-Powered Microfluidic Device | - |
dc.type | Article | - |
dc.identifier.wosid | 000533346400109 | - |
dc.identifier.scopusid | 2-s2.0-85083831133 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 8 | - |
dc.citation.publicationname | SENSORS | - |
dc.identifier.doi | 10.3390/s20082267 | - |
dc.contributor.localauthor | Park, Je-Kyun | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | colorimetric detection | - |
dc.subject.keywordAuthor | E | - |
dc.subject.keywordAuthor | coli O157:H7 | - |
dc.subject.keywordAuthor | finger-powered | - |
dc.subject.keywordAuthor | microfluidics | - |
dc.subject.keywordAuthor | size-based filtration | - |
dc.subject.keywordPlus | IMMUNOMAGNETIC SEPARATION | - |
dc.subject.keywordPlus | PATHOGENIC BACTERIA | - |
dc.subject.keywordPlus | SERS | - |
dc.subject.keywordPlus | ENUMERATION | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | O157-H7 | - |
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