Colorimetric Detection of Escherichia coli O157:H7 with Signal Enhancement Using Size-Based Filtration on a Finger-Powered Microfluidic Device

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dc.contributor.authorJo, Younggeunko
dc.contributor.authorPark, Juhwanko
dc.contributor.authorPark, Je-Kyunko
dc.date.accessioned2020-06-10T06:20:06Z-
dc.date.available2020-06-10T06:20:06Z-
dc.date.created2020-06-08-
dc.date.created2020-06-08-
dc.date.issued2020-04-
dc.identifier.citationSENSORS, v.20, no.8-
dc.identifier.issn1424-8220-
dc.identifier.urihttp://hdl.handle.net/10203/274590-
dc.description.abstractAlthough immunomagnetic separation is a useful sample pretreatment method that can be used to separate target pathogens from a raw sample, it is challenging to remove unbound free magnetic nanoparticles (MNPs) for colorimetric detection of target pathogens. Here, size-based filtration was exploited for the rapid on-site detection of pathogens separated by immunomagnetic separation in order to remove unbound free MNPs using a finger-powered microfluidic device. A membrane filter and an absorbent pad were integrated into the device and a mixture of unbound free MNPs and MNP-bound Escherichia coli (E. coli) O157:H7 was dispensed over the membrane filter by pressing and releasing the pressure chamber. A colorimetric signal was generated by MNP-bound E. coli O157:H7 while unbound free MNPs were washed out by the absorbent. Furthermore, the colorimetric signals can be amplified using a gold enhancer solution when gold-coated MNPs were used instead of MNPs. As a result, 10(2) CFU/mL E. coli O157:H7 could be detected by the enhanced colorimetric signal on a proposed device.-
dc.languageEnglish-
dc.publisherMDPI-
dc.titleColorimetric Detection of Escherichia coli O157:H7 with Signal Enhancement Using Size-Based Filtration on a Finger-Powered Microfluidic Device-
dc.typeArticle-
dc.identifier.wosid000533346400109-
dc.identifier.scopusid2-s2.0-85083831133-
dc.type.rimsART-
dc.citation.volume20-
dc.citation.issue8-
dc.citation.publicationnameSENSORS-
dc.identifier.doi10.3390/s20082267-
dc.contributor.localauthorPark, Je-Kyun-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorcolorimetric detection-
dc.subject.keywordAuthorE-
dc.subject.keywordAuthorcoli O157:H7-
dc.subject.keywordAuthorfinger-powered-
dc.subject.keywordAuthormicrofluidics-
dc.subject.keywordAuthorsize-based filtration-
dc.subject.keywordPlusIMMUNOMAGNETIC SEPARATION-
dc.subject.keywordPlusPATHOGENIC BACTERIA-
dc.subject.keywordPlusSERS-
dc.subject.keywordPlusENUMERATION-
dc.subject.keywordPlusSTRATEGIES-
dc.subject.keywordPlusO157-H7-
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