DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, Yoo-Kyoung | ko |
dc.contributor.author | Son, Sumin | ko |
dc.contributor.author | Choi, Yoonjoo | ko |
dc.contributor.author | Hwang, Da-Eun | ko |
dc.contributor.author | Seo, Hyo-Deok | ko |
dc.contributor.author | Lee, Joong-jae | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2020-05-26T06:20:26Z | - |
dc.date.available | 2020-05-26T06:20:26Z | - |
dc.date.created | 2020-05-25 | - |
dc.date.created | 2020-05-25 | - |
dc.date.created | 2020-05-25 | - |
dc.date.created | 2020-05-25 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.117, no.6, pp.1904 - 1908 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/274302 | - |
dc.description.abstract | Complement component 3a (C3a) plays a crucial role in the immune response and host defense, but it is also involved in pro-inflammatory responses, causing many inflammatory disorders. Blockade of C3a has been regarded as a potent therapeutic strategy for inflammatory diseases. Here, we present the development of a human C3a (hC3a)-specific protein binder, which effectively inhibits pro-inflammatory responses. The protein binder, which is composed of leucine-rich repeat modules, was selected against hC3a through phage display, and its binding affinity was matured up to 600 pM by further expanding the binding interface in a module-by-module manner. The developed protein binder was shown to have more than 10-fold higher specificity to hC3a compared with human C5a, exhibiting a remarkable suppression effect on pro-inflammatory response in monocyte, by blocking the interaction between hC3a and its receptor. The hC3a-specific protein binder is likely to have a therapeutic potential for C3a-mediated inflammatory diseases. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.title | Effective inhibition of C3a-mediated pro-inflammatory response by a human C3a-specific protein binder | - |
dc.type | Article | - |
dc.identifier.wosid | 000530930000025 | - |
dc.identifier.scopusid | 2-s2.0-85082964055 | - |
dc.type.rims | ART | - |
dc.citation.volume | 117 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 1904 | - |
dc.citation.endingpage | 1908 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.27309 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | anaphylatoxin | - |
dc.subject.keywordAuthor | C3a | - |
dc.subject.keywordAuthor | inflammatory disease | - |
dc.subject.keywordAuthor | protein binder | - |
dc.subject.keywordAuthor | protein engineering | - |
dc.subject.keywordAuthor | repebody | - |
dc.subject.keywordPlus | C3A | - |
dc.subject.keywordPlus | C5A | - |
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