DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Jea Sung | ko |
dc.contributor.author | Im, San Hae | ko |
dc.contributor.author | Kang, Yoo Kyung | ko |
dc.contributor.author | Kim, Yang Soo | ko |
dc.contributor.author | Chung, Hyun Jung | ko |
dc.date.accessioned | 2020-05-26T02:20:22Z | - |
dc.date.available | 2020-05-26T02:20:22Z | - |
dc.date.created | 2020-02-23 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | BIOMATERIALS SCIENCE, v.8, no.8, pp.2111 - 2119 | - |
dc.identifier.issn | 2047-4849 | - |
dc.identifier.uri | http://hdl.handle.net/10203/274291 | - |
dc.description.abstract | Gram-negative bacteria are a significant cause of infections acquired in both hospital and community settings, resulting in a high mortality rate worldwide. Currently, a Gram-negative infection is diagnosed by symptom evaluation and is treated with empiric antibiotics which target both Gram-negative and Gram-positive bacteria. A rapid and simple diagnostic method would enable immediate and targeted treatment, while dramatically reducing antibiotic overuse. Herein, we introduce a method utilizing a fluorescent derivative of colistin (COL-FL), that can directly label the Gram-negative cell wall of live bacteria and universally detect the targets within 10 min. By using the COL-FL assay, we achieved the differential labeling of various Gram-negative pathogens related to hospital-acquired infections, which could be subsequently detected via spectrofluorometry and microscopy. Further, we determined that our method can be used for complex samples, such as combinations of multiple bacterial types; bacteria in the presence of mammalian cells; and bacteria with serum components. This assay can be integrated into a simple diagnostic platform for rapid screening tests and the stratification of Gram-negative bacterial infections in the clinic. | - |
dc.language | English | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives | - |
dc.type | Article | - |
dc.identifier.wosid | 000530471400022 | - |
dc.identifier.scopusid | 2-s2.0-85083544733 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 2111 | - |
dc.citation.endingpage | 2119 | - |
dc.citation.publicationname | BIOMATERIALS SCIENCE | - |
dc.identifier.doi | 10.1039/c9bm01926j | - |
dc.contributor.localauthor | Chung, Hyun Jung | - |
dc.contributor.nonIdAuthor | Kim, Yang Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BLOOD-STREAM INFECTIONS | - |
dc.subject.keywordPlus | PATHOGENIC BACTERIA | - |
dc.subject.keywordPlus | POSITIVE BACTERIA | - |
dc.subject.keywordPlus | FLUORESCENT-PROBE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | TRENDS | - |
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