Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

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Gram-negative bacteria are a significant cause of infections acquired in both hospital and community settings, resulting in a high mortality rate worldwide. Currently, a Gram-negative infection is diagnosed by symptom evaluation and is treated with empiric antibiotics which target both Gram-negative and Gram-positive bacteria. A rapid and simple diagnostic method would enable immediate and targeted treatment, while dramatically reducing antibiotic overuse. Herein, we introduce a method utilizing a fluorescent derivative of colistin (COL-FL), that can directly label the Gram-negative cell wall of live bacteria and universally detect the targets within 10 min. By using the COL-FL assay, we achieved the differential labeling of various Gram-negative pathogens related to hospital-acquired infections, which could be subsequently detected via spectrofluorometry and microscopy. Further, we determined that our method can be used for complex samples, such as combinations of multiple bacterial types; bacteria in the presence of mammalian cells; and bacteria with serum components. This assay can be integrated into a simple diagnostic platform for rapid screening tests and the stratification of Gram-negative bacterial infections in the clinic.
Publisher
Royal Society of Chemistry
Issue Date
2020-04
Language
English
Article Type
Article
Citation

BIOMATERIALS SCIENCE, v.8, no.8, pp.2111 - 2119

ISSN
2047-4849
DOI
10.1039/c9bm01926j
URI
http://hdl.handle.net/10203/274291
Appears in Collection
NT-Journal Papers(저널논문)
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