(A) novel D-allose operon in escherichia coli K-12 and its secondary function as a D-ribose transporter = 대장균의 D-알로스 오페론과 D-리보스 수송기능

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A search for a gene responsible for a low-affinity transporter of D-ribose revealed that the Escherichia coli K-12 strain MC4100 utilizes D-allose as a sole carbon source by having D-allose transporter. The operon involved in D-allose metabolism was localized at 92.8 min of the E. coli linkage map. It consists of six genes which are inducible by D-allose and are under the control of the repressor gene alsR. This operon is also subject to catabolite repression. Three genes, alsB, alsA, and alsC, appear to be necessary for transport of D-allose as well as for transport of structurally related D-ribose at low affinity. D-allose-binding protein encoded by alsB is a periplasmic protein, which has an affinity to D-allose with a Kd of 0.33 micro M. The transport system has a feature of binding protein-mediated ABC-transporter which includes an ATP-binding component (AlsA). It was found that AlsE (a putative D-allulose-6-phosphate 3-epimerase), neither AlsK (a putative D-allose kinase) nor RpiB (a putative D-allose-6-phosphate isomerase), is necessary for allose metabolism. During this study, the strain W3110, a well-known wild type E. coli, was found to have a defect in the transport of D-allose mediated by the allose permease. It was observed that Escherichia coli can still grow on D-ribose in the absence of both the ribose and the allose permease. Futhermore, since several partially characterized mutations also affects the ribose growth (Rha-, xylA-, a locus linked to rbs operon), it seems that low-affinity D-ribose transport is mediated by a sugar specific system such as in the cases of D-xylose and L-arabinose which have their specific low-affinity transport systems.
Advisors
Park, Chan-Kyuresearcher박찬규researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135083/325007 / 000925089
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생물과학과, 1998.2, [ v, 132 p. b삽도 ; ]

Keywords

Metabolism; D-ribose; D-allose; Ribose low-affinity ansport; 리보스 저친화성 수송계; 대사; D-리보스; D-알로스

URI
http://hdl.handle.net/10203/27420
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135083&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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