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College of Life Science and Bioengineering(생명과학기술대학)Dept. of Biological Sciences(생명과학과)BS-Journal Papers(저널논문)

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

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dc.contributor.authorKarottki, Karen Julie la Courko
dc.contributor.authorHefzi, Hoomanko
dc.contributor.authorXiong, Kaiko
dc.contributor.authorShamie, Isaacko
dc.contributor.authorHansen, Anders Holmgaardko
dc.contributor.authorLi, Songyuanko
dc.contributor.authorPedersen, Lasse Ebdrupko
dc.contributor.authorLi, Shangzhongko
dc.contributor.authorLee, Jae Seongko
dc.contributor.authorLee, Gyun Minko
dc.contributor.authorKildegaard, Helene Faustrupko
dc.contributor.authorLewis, Nathan E.ko
dc.date.accessioned2020-05-13T08:20:07Z-
dc.date.available2020-05-13T08:20:07Z-
dc.date.created2019-12-02-
dc.date.issued2020-02-
dc.identifier.citationBIOTECHNOLOGY AND BIOENGINEERING, v.117, no.2, pp.593 - 598-
dc.identifier.issn0006-3592-
dc.identifier.urihttp://hdl.handle.net/10203/274174-
dc.description.abstractChinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced. This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.-
dc.languageEnglish-
dc.publisherWILEY-
dc.titleAwakening dormant glycosyltransferases in CHO cells with CRISPRa-
dc.typeArticle-
dc.identifier.wosid000495781500001-
dc.identifier.scopusid2-s2.0-85075214541-
dc.type.rimsART-
dc.citation.volume117-
dc.citation.issue2-
dc.citation.beginningpage593-
dc.citation.endingpage598-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOENGINEERING-
dc.identifier.doi10.1002/bit.27199-
dc.contributor.localauthorLee, Gyun Min-
dc.contributor.nonIdAuthorKarottki, Karen Julie la Cour-
dc.contributor.nonIdAuthorHefzi, Hooman-
dc.contributor.nonIdAuthorXiong, Kai-
dc.contributor.nonIdAuthorShamie, Isaac-
dc.contributor.nonIdAuthorHansen, Anders Holmgaard-
dc.contributor.nonIdAuthorLi, Songyuan-
dc.contributor.nonIdAuthorPedersen, Lasse Ebdrup-
dc.contributor.nonIdAuthorLi, Shangzhong-
dc.contributor.nonIdAuthorLee, Jae Seong-
dc.contributor.nonIdAuthorKildegaard, Helene Faustrup-
dc.contributor.nonIdAuthorLewis, Nathan E.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCHO-
dc.subject.keywordAuthorCRISPRa-
dc.subject.keywordAuthorglycosylation-
dc.subject.keywordAuthorMgat3-
dc.subject.keywordAuthorSt6gal1-
dc.subject.keywordPlusANTIBODIES-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusHOST-
dc.subject.keywordPlusCAS9-
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