DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Seonghui | ko |
dc.contributor.author | Shin, Heegwon | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.date.accessioned | 2020-03-23T08:20:20Z | - |
dc.date.available | 2020-03-23T08:20:20Z | - |
dc.date.created | 2019-08-30 | - |
dc.date.created | 2019-08-30 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | BMB REPORTS, v.53, no.2, pp.94 - 99 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | http://hdl.handle.net/10203/273387 | - |
dc.description.abstract | Brain cytoplasmic 200 RNA (BC200 RNA) is proposed to ad as a local translational modulator by inhibiting translation after being targeted to neuronal dendrites. However, the mechanism by which BC200 RNA inhibits translation is not fully understood. Although a detailed functional analysis of RNA motifs is essential for understanding the BC200 RNA-mediated translation-inhibition mechanism, there is little relevant research on the subject. Here, we performed a systematic domain-dissection analysis of BC200 RNA to identify functional RNA motifs responsible for its translational-inhibition activity. Various RNA variants were assayed for their ability to inhibit translation of luciferase mRNA in vitro. We found that the 111-200-nucleotide region consisting of part of the Alu domain as well as the A/C-rich domain (consisting of both the A-rich and C-rich domains) is most effective for translation inhibition. Surprisingly, we also found that individual A-rich, A/C-rich, and Alu domains can enhance translation but at different levels for each domain, and that these enhancing effects manifest as cap-dependent translation. | - |
dc.language | English | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.title | Functional analysis of RNA motifs essential for BC200 RNA-mediated translational regulation | - |
dc.type | Article | - |
dc.identifier.wosid | 000518179300005 | - |
dc.identifier.scopusid | 2-s2.0-85081109551 | - |
dc.type.rims | ART | - |
dc.citation.volume | 53 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 94 | - |
dc.citation.endingpage | 99 | - |
dc.citation.publicationname | BMB REPORTS | - |
dc.identifier.doi | 10.5483/BMBRep.2020.53.2.153 | - |
dc.identifier.kciid | ART002560618 | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | A/C-rich domain | - |
dc.subject.keywordAuthor | Alu domain | - |
dc.subject.keywordAuthor | BC200 RNA | - |
dc.subject.keywordAuthor | RNA motifs | - |
dc.subject.keywordAuthor | Translational regulation | - |
dc.subject.keywordPlus | LONG NONCODING RNAS | - |
dc.subject.keywordPlus | NEURAL BC1 RNA | - |
dc.subject.keywordPlus | POLY(A)-BINDING PROTEIN | - |
dc.subject.keywordPlus | NEURONAL BC1 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | LOCATION | - |
dc.subject.keywordPlus | SYSTEMS | - |
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