DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Pack, Moo-Young | - |
dc.contributor.advisor | 박무영 | - |
dc.contributor.author | Lee, Dong-Seok | - |
dc.contributor.author | 이동석 | - |
dc.date.accessioned | 2011-12-12T07:33:56Z | - |
dc.date.available | 2011-12-12T07:33:56Z | - |
dc.date.issued | 1987 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=61005&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27260 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생물공학과, 1987.2, [ xii, 125 p. ] | - |
dc.description.abstract | A substantial overproduction and secretion of endo-$\beta$-1,4-glucanase via a shuttle vector, pCK98, carrying a Bacillus subtilis gene coding for this enzyme was allowed in $\underline{Bacillus}$ $\underline{subtilis}$ and $\underline{B.}$ $\underline{megaterium}$. Activities of the exocellular enzymes produced by $\underline{B.}$ $\underline{subtilis}$ RM125 (pCK98) and $\underline{B.}$ $\underline{megaterium}$ (pCK98) were found to be higher than that produced by the original gene donor $\underline{B.}$ $\underline{subtilis}$ strain by 52- and 43-fold, respectively. Most of the enzyme was produced during the exponential growth period and the production was not repressed by glucose or cellobiose. The plasmid was stable in $\underline{B.}$ $\underline{megaterium}$ but not in $\underline{B.}$ $\underline{subtilis}$. The production of $\beta$-glucosidase via new shuttle vectors having a $\underline{Alcaligenes}$ $\underline{faecalis}$ gene encoding this enzyme was less effectively allowed in $\underline{Bacillus}$ and $\underline{E.}$ $\underline{coli}$ cells even though the gene was put on amplifiable plasmids. Contrary to the endo-$\beta$-1, 4-glucanase gene, the $\beta$-glucosidase gene was comparatively unstable in Bacillus and E. coli cells. On the analysis of plasmids isolated from resultant transformed cells, intactness, deletion, rearrangement or modification of $\beta$-glucosidase gene portion in shuttle plasmids were all found. The concomitant production of glucose from carboxymethyl cullulose (CMC) with $\beta$-glucosidase and endo- $\beta$-1,4-glucansase complex produced by $\underline{Bacillus}$ or $\underline{E.}$ $\underline{coli}$ transformants was observed depending on the comparatively long incubation period. Additionally, a novel procedure using polysaccharides and dyes for the rapid detection and comparison of various polysaccharase activities was established. The starch, CMC, lichenan, laminarin, or pustulan as substrates, together Congo Red... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Use of bacilli for overproduction and secretionof endo-β-1,4-glucanase and β-glucosidase encoded by cloned genes | - |
dc.title.alternative | 크론된 유전자에 의한 endo-β-1,4-glucanse와 β-glucosidase 의 Bacillus 숙주를 통한 과잉 생산 및 분비 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 61005/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000825217 | - |
dc.contributor.localauthor | Pack, Moo-Young | - |
dc.contributor.localauthor | 박무영 | - |
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