Studies on the immobilized urokinase = 고정화 유로키나제의 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 256
  • Download : 0
Plasminogen activators are serine proteases with trypsin-like specificity which convert the inactive zymogen plasminogen to enzymatically active plasmin and thereby initiate fibrinolysis $\mbox{\underline{in}}$ $\mbox{\underline{vitro}}$ and $\mbox{\underline{in}}$ $\mbox{\underline{vivo}}$. Among them, human urinary type plasminogen activator, known as urokinase (EC.3.4.99.26) has been used clinically for treatment of thrombosis and/or vascular obstructions. The half-life of urokinase $\mbox{\underline{in}}$ $\mbox{\underline{vivo}}$, however, is very short and hence large amount of urokinase and repeated injections are required to maintain enough fibrinolytic potential $\mbox{\underline{in}}$ $\mbox{\underline{vivo}}$, when urokinase is used for the clinical treatment. In addition, many prosthetic materials such as artificial organs are widely used. Because of development of thrombus on the surfaces of body prosthetic material contact to blood, continuous use in body has severe limitations and application of urokinase to artificial organs is gradually required. To improve these disadvantages and to develope clinical application of urokinase requirements to these fundamental scientific knowledge on urokinase, immobilization/conjugation were attempted in studying 1) model study of immobilization of urokinase on Sepharose matrix, 2) development of conjugated form of urokinase on soluble dextran, 3) development of immobilized urokinase on cellulose hollow fiber membrane used in artificial kidney set. Immobilization of urokinase on insoluble Sepharose gel was succeeded as follows ; To the CNBr-activated Sepharose gel, urokinase was directly coupled. The resulting urokinase showed 37\% caseinolytic activity compared with initial loading amount, and 14.4 CTA units of specific activity. And Sep-$C_n$-COOH-UK was prepared by coupling to NOS-activated Sep-($CH_2$)n_COOH which was previously synthetized from CNBr-activated Sepharose gel and $NH_2$-($CH_2$)n_CO...
Advisors
Byun, Si-Myung변시명
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1984
Identifier
60841/325007 / 000795071
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생물공학과, 1984.2, [ x, 148, [5] p. ]

URI
http://hdl.handle.net/10203/27243
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=60841&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0