DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ki-Hun | ko |
dc.contributor.author | Hwang, Jihyun | ko |
dc.contributor.author | Kim, Jin Hong | ko |
dc.contributor.author | Son, Kyung-Pyo | ko |
dc.contributor.author | Jang, Yejin | ko |
dc.contributor.author | Kim, Meehyein | ko |
dc.contributor.author | Kang, Suk-Jo | ko |
dc.contributor.author | Lee, Jie-Oh | ko |
dc.contributor.author | Kang, Jin Young | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.date.accessioned | 2020-03-19T01:24:02Z | - |
dc.date.available | 2020-03-19T01:24:02Z | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.created | 2020-01-07 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.citation | RNA BIOLOGY, v.17, no.3, pp.325 - 334 | - |
dc.identifier.issn | 1547-6286 | - |
dc.identifier.uri | http://hdl.handle.net/10203/272364 | - |
dc.description.abstract | Retinoic acid-inducible gene I (RIG-I) is responsible for innate immunity via the recognition of short double-stranded RNAs in the cytosol. With the clue that G-U wobble base pairs in the influenza A virus's RNA promoter region are responsible for RIG-I activation, we determined the complex structure of RIG-I Delta CARD and a short hairpin RNA with G-U wobble base pairs by X-ray crystallography. Interestingly, the overall helical backbone trace was not affected by the presence of the wobble base pairs; however, the base pair inclination and helical axis angle changed upon RIG-I binding. NMR spectroscopy revealed that RIG-I binding renders the flexible base pair of the influenza A virus's RNA promoter region between the two G-U wobble base pairs even more flexible. Binding to RNA with wobble base pairs resulted in a more flexible RIG-I complex. This flexible complex formation correlates with the entropy-favoured binding of RIG-I and RNA, which results in tighter binding affinity and RIG-I activation. This study suggests that the structure and dynamics of RIG-I are tailored to the binding of specific RNA sequences with different flexibility. | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.title | Structural and biophysical properties of RIG-I bound to dsRNA with G-U wobble base pairs | - |
dc.type | Article | - |
dc.identifier.wosid | 000503803800001 | - |
dc.identifier.scopusid | 2-s2.0-85076899855 | - |
dc.type.rims | ART | - |
dc.citation.volume | 17 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 325 | - |
dc.citation.endingpage | 334 | - |
dc.citation.publicationname | RNA BIOLOGY | - |
dc.identifier.doi | 10.1080/15476286.2019.1700034 | - |
dc.contributor.localauthor | Kang, Suk-Jo | - |
dc.contributor.localauthor | Lee, Jie-Oh | - |
dc.contributor.localauthor | Kang, Jin Young | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | Kim, Jin Hong | - |
dc.contributor.nonIdAuthor | Jang, Yejin | - |
dc.contributor.nonIdAuthor | Kim, Meehyein | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Retinoic acid-inducible gene I | - |
dc.subject.keywordAuthor | dsRNA | - |
dc.subject.keywordAuthor | wobble base pair | - |
dc.subject.keywordAuthor | x-ray crystallography | - |
dc.subject.keywordAuthor | hydrogen exchange rate | - |
dc.subject.keywordAuthor | differential scanning fluorimetry | - |
dc.subject.keywordAuthor | isothermal titration calorimetry | - |
dc.subject.keywordAuthor | entropy favoured binding | - |
dc.subject.keywordAuthor | antiviral immunity | - |
dc.subject.keywordPlus | INFLUENZA-A | - |
dc.subject.keywordPlus | RNA RECOGNITION | - |
dc.subject.keywordPlus | VIRUS PANHANDLE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | FEATURES | - |
dc.subject.keywordPlus | HAIRPINS | - |
dc.subject.keywordPlus | MDA5 | - |
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