DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sheet, Sunirmal | ko |
dc.contributor.author | Sathishkumar, Yesupatham | ko |
dc.contributor.author | Ghosh, Kuntal | ko |
dc.contributor.author | Choi, Mi-Sook | ko |
dc.contributor.author | Shim, Kwan Seob | ko |
dc.contributor.author | Lee, Yang Soo | ko |
dc.date.accessioned | 2020-02-11T05:20:06Z | - |
dc.date.available | 2020-02-11T05:20:06Z | - |
dc.date.created | 2020-02-10 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | ENZYME AND MICROBIAL TECHNOLOGY, v.133 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | http://hdl.handle.net/10203/272239 | - |
dc.description.abstract | The present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The results showed that LSMMG-cultivated cells had lower survival rate and lower D-values under heat and acid stress conditions compared to cells grown under normal gravity (NG). Interestingly, the cold resistance was elevated in cells cultivated under LSMMG conditions when compared to NG conditions. A higher amount of anteiso-branched chain fatty acids and lower ratio of iso/anteiso were observed in LSMMG cultured cells, which would contribute to increased membrane fluidity. Under LSMMG conditions, upregulated expression of cold stress-related genes (cspA, cspB, and cspD) was noticed but no change in expression was observed for heat (dnaK, groES, clpC, clpP, and clpE) and acid stress-related genes (sigB). The LSMMG-grown cells showed inferior virulence capacity in terms of infection, cell cycle arrest, and apoptosis induction in Caco-2 cells compared to those grown under NG conditions. Approximately 3.65, 2.13, 4.02, and 2.65-fold downregulation of prfA, hly, inlA, and bsh genes, respectively, in LSMMG-cultured cells might be the reason for reduced virulence. In conclusion, these findings suggest that growth under LSMMG conditions stimulates alterations in L. monocytogenes stress/virulence response, perhaps due to changes in lipid composition and related genes expression. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Modulatory effect of low-shear modeled microgravity on stress resistance, membrane lipid composition, virulence, and relevant gene expression in the food-borne pathogen Listeria monocytogenes | - |
dc.type | Article | - |
dc.identifier.wosid | 000508743300006 | - |
dc.identifier.scopusid | 2-s2.0-85074172271 | - |
dc.type.rims | ART | - |
dc.citation.volume | 133 | - |
dc.citation.publicationname | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.enzmictec.2019.109440 | - |
dc.contributor.nonIdAuthor | Sheet, Sunirmal | - |
dc.contributor.nonIdAuthor | Ghosh, Kuntal | - |
dc.contributor.nonIdAuthor | Choi, Mi-Sook | - |
dc.contributor.nonIdAuthor | Shim, Kwan Seob | - |
dc.contributor.nonIdAuthor | Lee, Yang Soo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Listeria monocytogenes | - |
dc.subject.keywordAuthor | Low-Shear modeled microgravity | - |
dc.subject.keywordAuthor | Stress response | - |
dc.subject.keywordAuthor | Membrane lipid composition | - |
dc.subject.keywordAuthor | Virulence | - |
dc.subject.keywordAuthor | Cell cycle | - |
dc.subject.keywordPlus | FATTY-ACID-COMPOSITION | - |
dc.subject.keywordPlus | O157H7 ATCC 35150 | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | HEAT-RESISTANCE | - |
dc.subject.keywordPlus | NEGATIVE REGULATOR | - |
dc.subject.keywordPlus | GROWTH TEMPERATURE | - |
dc.subject.keywordPlus | 1ST GENE | - |
dc.subject.keywordPlus | SHOCK | - |
dc.subject.keywordPlus | SALMONELLA | - |
dc.subject.keywordPlus | ALTERS | - |
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