(A) cell electroporation and viability monitoring chip using a single channel with multiple electric field zones = 다수의 전기장 분포가 생성되는 단일 미세유로를 이용한 세포 전기천공 및 활성도 분석칩

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This thesis presents the cell electroporation (EP) and viability monitoring chips using a single channel with multiple electric field zones to find the optimum cell EP and viability conditions for stable cell transfection. The previous chips utilize one cell chamber with integrated multiple electrodes or multiple parallel microchannels with a pair of external electrodes to form multiple electric fields, thus resulting in complex structure. Therefore, we present two different types of single-microchannel cell EP and viability monitoring chips using the multiple electric field zones generated by a pair of external electrodes across stepwise (prototype S) and tapered (proto-type T) channel widths; thus making it possible to find the optimum cell EP rate and viability conditions for stable cell transfection. The present cell EP rate and viability monitoring chips have a single microchannel of stepwise (proto-type S) and tapered (prototype T) widths between an inlet and an outlet port. After seeding cells in the micro-channel, we supplied a pulse signal to the electrodes plugged in the inlet and outlet ports; generating multiple electric field zones along the microchannel by channel width change in a single channel. The stepwise widths of prototype S were designed as 200, 222, 250, 286, and 334μm so that 5 discrete electric fields (0.3, 0.35, 0.4, 0.45, 0.5kV/cm) are generated in the single channel at the supplied voltage of 340V. The tapered widths of prototype T were designed to change from 200μm to 334μm so that continuous electric field (0.3~0.5kV/cm) is formed at the voltage of 319V. And then, we observed the EP rate and viability of cells in the multiple electric field zones of the single channel by staining the cells with Propidium Iodide and Calcein AM, respectively. The present cell EP and viability monitoring chips were fabricated by PDMS molding process. In the experimental study, we used H23 and A549 human lung cancer cell lines to characterize the EP r...
Advisors
Cho, Young-Horesearcher조영호researcher
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2011
Identifier
467643/325007  / 020093070
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2011.2, [ v, 38 p. ]

Keywords

viability; electroporation (EP); multiple electric field; 다수의 전기장; 활성도; 세포 전기천공

URI
http://hdl.handle.net/10203/27208
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=467643&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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