Magnetophoretic label-free cell separation using paramagnetic solution in a microchannel = 미세채널 내에서 상자성 용액을 이용한 자기영동 기반 비표지 방식의 세포분리

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This thesis presents a diamagnetic repulsion magnetophoresis system for enhancing separation effect of different size particles by tuning the magnetic susceptibility of medium using paramagnetic solution. To demonstrate this system, various concentrations of paramagnetic solution were applied to separate 8- and 10-$\mum$ polystyrene beads in a microfluidic channel with a magnetic field. As the concentration of paramagnetic solution increased, the distance between two different beads was widened as well as the deflection of those beads became larger under the magnetic field. For cell separation, red blood cells and U937 cells were applied with various concentrations of paramagnetic solution. By using 40 mM gadolinium diethylenetriaminepentaacetic acid solution, we have achieved label-free separation of U937 cells from red blood cells using paramagnetic solution. The separation purity and throughput was 90 % and $1\times10^{5}$ cells/h, respectively. This new approach would be a powerful tool for label-free separation of rare cells and for multi-target separation with high resolution.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
455151/325007  / 020084180
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2010.08, [ viii, 51 p. ]

Keywords

label-free; paramagnetic solution; magnetophoresis; 자기영동; 비표지; 상자성 용액

URI
http://hdl.handle.net/10203/27194
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=455151&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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