Microfluidic in vitro culture system mimicking peristaltic stimulation in bovine embryos = 미세유체기술을 기반으로 연동자극을 모사한 소 수정란 체외배양시스템

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This work demonstrates a novel microfluidic $\It{in vitro}$ cultivation system for embryos that improves their development using a partially constricted channel that mimics peristaltic muscle contraction (the gravity-driven stimulation system). To investigate the compressive effects of constriction geometry on embryonic development, different constriction widths of the channel were designed. Bovine embryos were loaded on the channel and the whole system was incubated on a tilting machine to provide embryo movement via gravity. The fertilized embryos were cultivated on the gravity-driven stimulation system until the blastocyst, hatching, or hatched blastocyst stages. The proportion of eight-cell development among total embryos in the constricted channel ($56.77\plusmn13.7%; mean\plusmnSD$) was superior to that in the straight channel ($23.97\plusmn11.0%$). Moreover, the membrane based mechanical stimulation was also designed because of needs for independent control of flow and compressive force (the pressure-driven stimulation system). Therefore, the possibility to improve developmental ratio for bovine embryos was suggested as supporting more precise$\It{in vivo}$ like environment.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
418905/325007  / 020083227
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2010.2, [ ix, 55 p. ]

Keywords

소; 연동운동; 체외배양; 미세유체; 수정란; embryo; bovine; peristalsis; IVC; Microfluidic

URI
http://hdl.handle.net/10203/27186
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=418905&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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