#### (A) Multicellular spheroid formation and extraction chip using removable cell trapping barriers = 제거 가능한 세포 포집 구조물을 이용한 다세포군집 형성 및 추출칩

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This thesis presents a spheroid chip for multicellular spheroid formation and extraction using removable cell trapping barriers. The previous chips using fixed trapping barriers have faced difficulties in extracting the spheroids. The present spheroid chip achieves a simple spheroid extraction using removable cell trapping barriers formed by membrane inflation. The membrane between a top layer and a bottom layer of the spheroid chip is inflated by the membrane pressure to form cell trapping barriers in the bottom layer. The cell trapping barriers hold cells to be filled in the trapping region to form spheroids. At the reduced membrane pressure, the deflated membrane removes the cell trapping barriers, allowing spheroids to exit to the spheroid outlet. A 4 × 1 array of the spheroid chips has been designed, fabricated and characterized. The cell trapping barriers, formed at the membrane pressure of 50kPa, hold the cells in the trapping region at an initial cell inlet pressure of 200Pa. After 24 hour incubation, the cell-cell interaction makes the trapped cells form the spheroids. The spheroids have been extracted through the spheroid outlet at the cell inlet pressure of 5kPa for the membrane pressure of 0kPa. The spheroids have the diameter of 197.2±11.7 $\mu m$, showing the viable cell percentage of 75.5% after extraction. It is demonstrated that the spheroid chips perform uniform formation and secure extraction of the spheroid for the next stage of spheroid processing.
Cho, Young-Horesearcher조영호researcher
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2009
Identifier
327260/325007  / 020074207
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2009. 8., [ vi, 60 p. ]

Keywords

Multicellular spheroid; Microfluidics; Cell trapping; 다세포군집; 마이크로플루이딕스; 세포포집; Multicellular spheroid; Microfluidics; Cell trapping; 다세포군집; 마이크로플루이딕스; 세포포집

URI
http://hdl.handle.net/10203/27179