Development of microchannel device for long term neuron culture = 신경세포의 장기간 배양을 위한 마이크로 채널 개발

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Micro technology holds the promise for advanced cell culture systems which can better mimic the in vivo environment and serve as good disease models. This advantage of micro technology can be leveraged to culture nerve cells in vitro in confined space and nanolitres of culture media. In this work, a tubeless no-flow condition microchannel device is developed with the capabilities to sustain in vitro neural culture for long periods of time. Primary hippocampal neurons from E-18 Sprague-Dawley (SD) rat are cultured inside poly-(dimethylsiloxane) (PDMS) microchannel device assembled on Poly-D-Lysine (PDL) coated substrate. Neurons are cultured inside closed microchannels of variable geometry for over 1 month in serum free media condition. The neuron culture is characterized based on different parameters and effect of channel geometry on neuron development is investigated. Further, this microchannel neuron culture device is integrated with Multielectrode array (MEA) for functional characterization and spontaneous extracellular signals are successfully recorded from neurons inside the channels. This microchannel neuron culture device can help to probe and manipulate the neuron microenvironment and serve as good tool for neuron circuit design, neuron based drug screening assays, neural tissue engineering and in vitro neurodegenerative disease models.
Advisors
Nam, Yoon-Keyresearcher남윤기researcher
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2009
Identifier
308630/325007  / 020074047
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2009.2, [ viii, 56 p. ]

Keywords

neuron culture; microchannels; multielectrode array; 신경세포 배양; 마이크로 채널; 다채널 전극; neuron culture; microchannels; multielectrode array; 신경세포 배양; 마이크로 채널; 다채널 전극

URI
http://hdl.handle.net/10203/27160
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=308630&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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