Microfluidic immunoassay for detection of allergen-specific IgE in an enhanced magnetic field gradient = 강화된 자기장 구배하에서 알레르겐 특이 항체 검출을 위한 미세 유체 기반의 면역 측정

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This paper reports a novel immunoassay method using superparamagnetic nanoparticles and an enhanced magnetic field gradient in a microfluidic device. In this detection scheme, microbeads are deflected from their own pathway as much as analytes are conjugated with microbeads and superparamagnetic nanoparticles by sandwich immunoreactions. Since the deflection velocity of microbeads is proportional to the magnetic field gradient, lower detection limit can be controlled by the higher magnetic field gradient. In this study, a ferromagnetic microstructure connected with a permanent magnet is used to increase the magnetic field gradient $(~10^4 T m^{-1})$ in a microfluidic device. The microfluidic device with the microchannel is fabricated by poly(dimethylsiloxane) (PDMS) molding process and bonded on the glass wafer with the electroplated Ni microstructure. We successfully apply this method to detect an allergen-specific IgE antibody in human serum. House dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, are used as the sources for allergen. For allergen-specific IgE detection, we use fluorescent polystyrene beads conjugated with two types of mite allergens, superparamagnetic nanoparticles conjugated with anti-human IgE, and mite-specific IgE antibodies. The detectable range of rabbit IgG is 200 pg $mL^{-1}$ to 1 pg $mL^{-1}$, and those of the mite-specific human IgE are 19.47 ng $mL^{-1}$ to 104 pg $mL^{-1}$ (D. pteronyssinus) and 10.68 ng $mL^{-1}$ to 151 pg $mL^{-1}$ (D. farinae), respectively. With this platform technology, the multiplexed immunoassay could be possible when the specific allergen proteins are immobilized with the fluorescently encoded microbeads.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오시스템학과,
Publisher
한국과학기술원
Issue Date
2006
Identifier
255488/325007  / 020043647
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2006.2, [ xi, 66 p. ]

Keywords

immunoassay; Microfluidics; magnetic nanoparticles; 알레르겐 특이 항체; 나노자성체; 미세 유체 기반의 면역 측정; allergen-specific IgE

URI
http://hdl.handle.net/10203/27118
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=255488&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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