Cell deformability monitoring chips based on orifice length dependent lysis rates = 미소유체 통로의 길이에 따른 통과세포의 파괴율을 바탕으로 한 변형성 검사칩에 관한 연구

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This thesis presents a novel method and device for cell deformability monitoring based on the cell lysis rates through a filter array having gradually increased orifice length. Compared to the conventional filtration, the present method is insensitive to the cell volume deviation since the lysis rate is dependent on the ratio of cell surface area to volume. The present method also provides simple and inexpensive system by eliminating optical components. The present cell deformability monitoring chips consist of four filters with variable orifice lengths with electrical sensing zones located before and after each filter. We design and fabricate three types of devices with 3 different orifice lengths: 2, 6.7, 11.2, 16μm for T-device, 2, 4.5, 6.7, 9μm for L-device and 9, 11.2, 13.6, 16μm for U-device. Since the tension of the cell membrane passing through the filters is proportional to the orifice length, each device types impose various tensions. Devices are fabricated by photolithography and PDMS molding process. In the experimental study, we use normal and chemically treated erythrocytes to compare the lysis rate depending of cell deformability. The maximum differences of lysis rate between normal and chemically treated erythrocytes are measured as 21.2 3.5% in the 6.7μm long orifice of T-devices, and 16.6 4.6% in the 4.5μm long orifice of L-devices. We estimate that the orifice length range of 4~7μm effectively discriminate the deformability difference of erythrocytes. For an improved accuracy of the cell deformability measurement, we suggest a modified design preventing plugging of the erythrocytes. In this work, we verify that the present cell deformability monitoring chips have potentials for quantifying the deformability of the cells in different physical condition.
Cho, Young-Horesearcher조영호researcher
한국과학기술원 : 바이오시스템학과,
Issue Date
249600/325007  / 020033931

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2005.8, [ [vii], 55 p. ]


cell filter array; cell lysis rate; Cell deformability; erythrocyte deformability; 적혈구 변형성; 세포 필터 어레이; 세포 파괴율; 세포 변형성

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