Microchip-based electrochemical immunoassay with surface-functionalized polydimethysiloxane channel = 폴리디메틸실록산 채널 표면을 기능화 시킨 미세칩을 이용한 전기화학적 면역측정

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This thesis reports a microchip-based electrochemical immunoassay with surface-functionalized poly(dimethylsiloxane) (PDMS) channel. While the standard microtiter plate-based immunoassay has required a long incubation time, large sample volumes, and tedious labors, the microchip-based immunoassay gives a solution against those drawbacks by integration of analytical sequences in microscale. In this study, a 3-electrode system for electrochemical detection, fabricated on a glass substrate by photolithography, was assembled to the PDMS channel by plasma treatment. For the effective immobilization of target antibodies, the internal surface of the PDMS channel was chemically modified into the silane monolayer containing the poly(ethyleneglycol) groups, which minimized non-specific binding. Additionally, the low height (50㎛) of the channel enables a rapid immunoassay by reducing the incubation time to 1 min. We chose the mouse IgG as a model analyte for sandwich immunoassay. A low detection limit of 485pg/mL was obtained at 95% confidence level. Furthermore, liquid handling was controlled by a syringe pump preserving a constant channel volume (0.7μL), which was free from tedious separation steps and erroneous volume control. Such an on-chip integration of immunological and enzymatic reactions, amperometric detection, and microfluidic separation system allows performing immunoassays more rapidly, easily, and economically.
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오시스템학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
243765/325007  / 020033558
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2005.2, [ ix, 72 p. ]

Keywords

immunoassay; electrochemistry; Microchip; surface-functionalization; 표면기능화; 면역측정; 전기화학; 미세칩

URI
http://hdl.handle.net/10203/27107
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=243765&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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