DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Dong-Sup | - |
dc.contributor.advisor | 김동섭 | - |
dc.contributor.author | Han, Sang-Jo | - |
dc.contributor.author | 한상조 | - |
dc.date.accessioned | 2011-12-12T07:28:11Z | - |
dc.date.available | 2011-12-12T07:28:11Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=240424&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/27102 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2004.8, [ iii, 21 p. ] | - |
dc.description.abstract | Quantitative RT-PCR methods are usually being used to overcome drawbacks of microarray technology caused by variability and detection of low expression level. Primer design is very critical step in RT-PCR methods to guarantee specificity and efficiency of a target amplicon. However, most of traditional primer design programs suggest primers on a single template of limited genetic complexity. In online RT-PCR primer databases, they contain the validated primers for small fraction of whole genes at present. To provide researchers with enough specific RT-PCR primer pairs for whole genes in Arabidopsis, we constructed and evaluated genome-wide primer-pairs database, which is called AtRTPrimer, considering homogeneous physical and chemical properties of each primer (homogeneity) of a gene, non-specific binding against all known genes (specificity), and another possible amplicons from its corresponding genomic DNA or similar cDNA (noise). This database is designed for a couple of types of quantitative RT-PCR: roughly speaking, conventional RT-PCR and real-time RT-PCR. Therefore, experimentalists can find out the appropriate set of pre-designed primer-pairs of their target genes to perform the desired type of RT-PCR using filtering conditions. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 프라이머 디자인 | - |
dc.subject | DILUTION WITH PROGRAMMABLE RATIO | - |
dc.subject | 데이타베이스 | - |
dc.subject | 애기장대합 | - |
dc.subject | 희석비율 조절맑벼학적 검출법 | - |
dc.subject | RT-PCR | - |
dc.subject | PRIMER DESIGN | - |
dc.subject | RT-PCR | - |
dc.subject | DATABASE | - |
dc.subject | AARABIDOPSISDILUTION | - |
dc.subject | TO-AND-FRO MIXING | - |
dc.title | AtRTPrimer | - |
dc.title.alternative | 애기장대용 RT-PCR 프라이머 데이타베이스 구축 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 240424/325007 | - |
dc.description.department | 한국과학기술원 : 바이오시스템학과, | - |
dc.identifier.uid | 020033666 | - |
dc.contributor.localauthor | Kim, Dong-Sup | - |
dc.contributor.localauthor | 김동섭 | - |
dc.title.subtitle | arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs database | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.