AtRTPrimer : arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs database = 애기장대용 RT-PCR 프라이머 데이타베이스 구축arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs database

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Quantitative RT-PCR methods are usually being used to overcome drawbacks of microarray technology caused by variability and detection of low expression level. Primer design is very critical step in RT-PCR methods to guarantee specificity and efficiency of a target amplicon. However, most of traditional primer design programs suggest primers on a single template of limited genetic complexity. In online RT-PCR primer databases, they contain the validated primers for small fraction of whole genes at present. To provide researchers with enough specific RT-PCR primer pairs for whole genes in Arabidopsis, we constructed and evaluated genome-wide primer-pairs database, which is called AtRTPrimer, considering homogeneous physical and chemical properties of each primer (homogeneity) of a gene, non-specific binding against all known genes (specificity), and another possible amplicons from its corresponding genomic DNA or similar cDNA (noise). This database is designed for a couple of types of quantitative RT-PCR: roughly speaking, conventional RT-PCR and real-time RT-PCR. Therefore, experimentalists can find out the appropriate set of pre-designed primer-pairs of their target genes to perform the desired type of RT-PCR using filtering conditions.
Advisors
Kim, Dong-Supresearcher김동섭researcher
Description
한국과학기술원 : 바이오시스템학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
240424/325007  / 020033666
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2004.8, [ iii, 21 p. ]

Keywords

프라이머 디자인; DILUTION WITH PROGRAMMABLE RATIO; 데이타베이스; 애기장대합; 희석비율 조절맑벼학적 검출법; RT-PCR; PRIMER DESIGN; RT-PCR; DATABASE; AARABIDOPSISDILUTION; TO-AND-FRO MIXING

URI
http://hdl.handle.net/10203/27102
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=240424&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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