(A) study on the enzyme assay in microfluidic channels = 미세 유체 채널을 이용한 효소 활성도 분석 기술에 대한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 284
  • Download : 0
A novel platform technology of enzyme assay using microfluidic channels was developed. The microfluidic device for alkaline phosphatase assay was fabricated by micromolding processes with PDMS (polydimethylysiloxane) polymer. This microfluidic device can be provided a novel assay platform which can overcome limitation of the conventional assay platform, which contains the requirement of special detection instrument, manual dilution of the substrate solution and individual measurement of enzyme reactions. In this study, ELISA reader instrument was used as a detection system and the substrate solution of different concentration was generated by microfluidic channel network. Enzyme reactions were measured in ELISA reader instrument, which occurred in six reaction chambers containing each different concentration of substrate solution. Reaction chambers also functioning detection chambers has been arranged in 96-well formats to be operated by ELISA reader. Overall dimension of the microfluidic device was the same size as a 96-well microplate. Enzyme activity was determined with the continuous methods that are generally an instantaneous observation, which was measured by ELISA reader instrument. Also the enzyme activity is possible to be estimated by stop method. In this thesis, stop method of enzyme assay was used as a standard assay, which showed error bars of less than ±1.7% enzyme activity. Employing the patterns of microfluidic channels, the substrate solution was diluted in serial and the enzyme solution was injected into the detection wells simultaneously. Dilution effect was examined by red food dye with error bars of less than ±9.43% absorbance and the experimental results have difference from the predicted value less than 1.82 % relative absorbance. The predicted values are calculated by the concentration equation derived in this paper. Initial velocity of enzyme reaction is measurable soon after enzyme and substrate solution is mixed together at six detect...
Advisors
Park, Je-Kyunresearcher박제균researcher
Description
한국과학기술원 : 바이오시스템학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
238642/325007  / 020023904
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오시스템학과, 2004.2, [ xii, 62 p. ]

Keywords

미세 유체 채널; 효소 활성도; MICROFLUIDIC CHANNELS; ENZYME ASSAY

URI
http://hdl.handle.net/10203/27092
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=238642&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0