DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jaehoon | ko |
dc.date.accessioned | 2019-12-31T02:21:17Z | - |
dc.date.available | 2019-12-31T02:21:17Z | - |
dc.date.created | 2019-12-28 | - |
dc.date.issued | 2019-12-04 | - |
dc.identifier.citation | 16th Asian Conference on Transcription | - |
dc.identifier.uri | http://hdl.handle.net/10203/270834 | - |
dc.description.abstract | RNF20/40 E3 ubiquitin ligase-mediated histone H2B monoubiquitylation plays important roles in many cellular processes, including transcriptional regulation. However, the multiple defects observed in RNF20-depleted cells suggest additional ubiquitylation targets of RNF20/40 beyond histone H2B. Here, using biochemically defined assays employing purified factors and cell-based analyses, we demonstrate that RNF20/40, in conjunction with its cognate E2 ubiquitin-conjugating enzyme RAD6, monoubiquitylates lysine 381 of eEF1BL, a heat shock transcription factor. Notably, monoubiquitylation of eEF1BL increases eEF1BL accumulation and potentiates recruitment of p-TEFb to the promoter regions of heat shock-responsive genes, leading to enhanced transcription of these genes. We further demonstrate that cooperative physical interactions among eEF1BL, RNF20/40, and HSF1 synergistically promote expression of heat shock-responsive genes. In addition to identifying eEF1BL as a novel ubiquitylation target of RNF20/40 and elucidating its function, we provide a molecular mechanism for the cooperative function of distinct transcription factors in heat shock-responsive gene transcription. | - |
dc.language | English | - |
dc.publisher | Asian Conference on Transcription | - |
dc.title | Regulation of heat shock-responsive gene transcription by RNF20/40-mediated eEF1BδLmonoubiquitylation | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.citation.publicationname | 16th Asian Conference on Transcription | - |
dc.identifier.conferencecountry | NZ | - |
dc.identifier.conferencelocation | Dunedin | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
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