Klf2-mediated recruitment of Polycomb Repressive Complex 1 is required for endoderm differentiation in mouse embryonic stem cells

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Polycomb repressive complex 1 (PRC1) plays important roles in expression of development-related genes, but precisely how PRC1 can be recruited to specific genomic loci remains unclear. Here, we show that DNA-binding transcription factor Krűppel-like factor 2 (Klf2) directly binds to Ring1b, a PRC1 subunit that has histone H2A ubiquitylation activity, and is responsible for recruitment of PRC1 to the promoter regions of several endoderm specific genes in mouse embryonic stem cells (mESCs). Klf2-deficient mESCs fail to downregulate expression of endoderm specific genes. Consistently, RNA-seq and cell differentiation analyses reveal that loss of Klf2 and Ring1b increases expression of the endoderm specific genes and facilitates endoderm differentiation. Collectively, our results suggest that Klf2 recruits PRC1 to the target genes and that they play roles in regulation of lineage specification and maintenance of ESC identity.
Publisher
한국분자세포생물학회
Issue Date
2019-12-11
Language
English
Citation

2019 한국분자세포생물학회 에피유전체학분과 심포지움

URI
http://hdl.handle.net/10203/270602
Appears in Collection
BS-Conference Papers(학술회의논문)
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