Regulation of heat shock-responsive gene transcription by RNF20/40-mediated eEF1BδLmonoubiquitylation

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 70
  • Download : 0
DC FieldValueLanguage
dc.identifier.citation2019 한국분자세포생물학회 단백질대사분과 심포지움-
dc.description.abstractRNF20/40 E3 ubiquitin ligase-mediated histone H2B monoubiquitylation plays important roles in many cellular processes, including transcriptional regulation. However, the multiple defects observed in RNF20-depleted cells suggest additional ubiquitylation targets of RNF20/40 beyond histone H2B. Here, using biochemically defined assays employing purified factors and cell-based analyses, we demonstrate that RNF20/40, in conjunction with its cognate E2 ubiquitin-conjugating enzyme RAD6, monoubiquitylates lysine 381 of eEF1BL, a heat shock transcription factor. Notably, monoubiquitylation of eEF1BL increases eEF1BL accumulation and potentiates recruitment of p-TEFb to the promoter regions of heat shock-responsive genes, leading to enhanced transcription of these genes. We further demonstrate that cooperative physical interactions among eEF1BL, RNF20/40, and HSF1 synergistically promote expression of heat shock-responsive genes. In addition to identifying eEF1BL as a novel ubiquitylation target of RNF20/40 and elucidating its function, we provide a molecular mechanism for the cooperative function of distinct transcription factors in heat shock-responsive gene transcription.-
dc.titleRegulation of heat shock-responsive gene transcription by RNF20/40-mediated eEF1BδLmonoubiquitylation-
dc.citation.publicationname2019 한국분자세포생물학회 단백질대사분과 심포지움-
dc.identifier.conferencelocation강원도 삼척 대명리조트 솔비치 그랜드 볼룸-
Appears in Collection
BS-Conference Papers(학술회의논문)
Files in This Item
There are no files associated with this item.


  • mendeley


rss_1.0 rss_2.0 atom_1.0