Monitoring of microbial reactions in subsurface has drawn significant interest in various bio-geotechnical engineering. In this research, the complex electrical conductivity responses was measured using induced polarization (IP) method during microbial growth in sand column. As a result, there were reduction of real conductivity and significant increment in imaginary conductivity. Also, the complex conductivity responses showed the relaxation behavior, proposed by the Cole-Cole model. The relaxation time of the response decreased in power function and the chargeability linearly increased and these coefficients were well matched with the bulk imaginary conductivity. According to the research, it appeared that the complex conductivity can effectively capture bacterial growth at the pore scale.