DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Hang Soo | ko |
dc.contributor.author | Jo, Jin Chul | ko |
dc.contributor.author | Shin, Chang-Hun | ko |
dc.contributor.author | Lee, Namil | ko |
dc.contributor.author | Choi, Joon-Sun | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Roe, Jung-Hye | ko |
dc.contributor.author | Kim, Chan-Wha | ko |
dc.contributor.author | Kwon, Ho Jeong | ko |
dc.contributor.author | Yoon, Yeo Joon | ko |
dc.date.accessioned | 2019-09-10T05:20:02Z | - |
dc.date.available | 2019-09-10T05:20:02Z | - |
dc.date.created | 2019-09-09 | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.46, no.8, pp.1205 - 1215 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.uri | http://hdl.handle.net/10203/267447 | - |
dc.description.abstract | Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximately 25%, producing similar to 4.95 g/L of CA, over the OR strain in the flask culture. Moreover, overexpression of the pathway-specific positive regulatory genes, ccaR and claR, in the OR strain improved CA yield by approximately 43% (similar to 5.66 g/L) in the flask. However, co-expression of the intact cas1 with ccaR-claR did not further improve CA production. In the 7 L fermenter culture, maximum CA production by the OR strain expressing the wild-type cas1 and ccaR-claR reached approximately 5.52 g/L and 6.01 g/L, respectively, demonstrating that reverse engineering or simple rational metabolic engineering is an efficient method for further improvement of industrial strains. | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain | - |
dc.type | Article | - |
dc.identifier.wosid | 000481796500014 | - |
dc.identifier.scopusid | 2-s2.0-85067066994 | - |
dc.type.rims | ART | - |
dc.citation.volume | 46 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1205 | - |
dc.citation.endingpage | 1215 | - |
dc.citation.publicationname | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s10295-019-02196-0 | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Cho, Hang Soo | - |
dc.contributor.nonIdAuthor | Jo, Jin Chul | - |
dc.contributor.nonIdAuthor | Shin, Chang-Hun | - |
dc.contributor.nonIdAuthor | Lee, Namil | - |
dc.contributor.nonIdAuthor | Choi, Joon-Sun | - |
dc.contributor.nonIdAuthor | Roe, Jung-Hye | - |
dc.contributor.nonIdAuthor | Kim, Chan-Wha | - |
dc.contributor.nonIdAuthor | Kwon, Ho Jeong | - |
dc.contributor.nonIdAuthor | Yoon, Yeo Joon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Clavulanic acid | - |
dc.subject.keywordAuthor | Streptomyces clavuligerus | - |
dc.subject.keywordAuthor | Reverse engineering | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | OVERPRODUCTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DNA | - |
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