DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cui, Chang-hao | ko |
dc.contributor.author | Jeon, Byeong-Min | ko |
dc.contributor.author | Fu, Yaoyao | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2019-09-03T05:20:10Z | - |
dc.date.available | 2019-09-03T05:20:10Z | - |
dc.date.created | 2019-09-02 | - |
dc.date.created | 2019-09-02 | - |
dc.date.created | 2019-09-02 | - |
dc.date.created | 2019-09-02 | - |
dc.date.created | 2019-09-02 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.103, no.17, pp.7003 - 7015 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | http://hdl.handle.net/10203/266605 | - |
dc.description.abstract | Use of recombinant glycosidases is a promising approach for the production of minor ginsenosides, e.g., Compound K (CK) and F-1, which have potential applications in the food industry. However, application of these recombinant enzymes for food-grade preparation of minor ginsenosides are limited by the lack of suitable expression hosts and low productivity. In this study, Corynebacterium glutamicum ATCC13032, a GRAS strain that has been used extensively for the industrial-grade production of additives for foodstuffs, was employed to express a novel beta-glucosidase (MT619) from Microbacterium testaceum ATCC 15829 with high ginsenoside-transforming activity. A cellulose-binding module was additionally fused to the N-terminus of MT619 for immobilization on cellulose, which is an abundant and safe material. Via one-step immobilization, the fusion protein in cell lysates was efficiently immobilized on regenerated amorphous cellulose at a high density (maximum 984 mg/g cellulose), increasing the enzyme concentration by 286-fold. The concentrated and immobilized enzyme showed strong conversion activities against protopanaxadiol- and protopanaxatriol-type ginsenosides for the production of CK and F-1. Using gram-scale ginseng extracts as substrates, the immobilized enzyme produced 7.59 g/L CK and 9.42 g/L F-1 in 24 h. To the best of our knowledge, these are the highest reported product concentrations of CK and F-1, and this is the first time that a recombinant enzyme has been immobilized on cellulose for the preparation of minor ginsenosides. This safe, convenient, and efficient production method could also be effectively exploited in the preparation of food-processing recombinant enzymes in the pharmaceutical, functional food, and cosmetics industries. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.title | High-density immobilization of a ginsenoside-transforming beta-glucosidase for enhanced food-grade production of minor ginsenosides | - |
dc.type | Article | - |
dc.identifier.wosid | 000480552400010 | - |
dc.identifier.scopusid | 2-s2.0-85068825339 | - |
dc.type.rims | ART | - |
dc.citation.volume | 103 | - |
dc.citation.issue | 17 | - |
dc.citation.beginningpage | 7003 | - |
dc.citation.endingpage | 7015 | - |
dc.citation.publicationname | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s00253-019-09951-4 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Cui, Chang-hao | - |
dc.contributor.nonIdAuthor | Fu, Yaoyao | - |
dc.contributor.nonIdAuthor | Im, Wan-Taek | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Compound K | - |
dc.subject.keywordAuthor | Ginsenoside F-1 | - |
dc.subject.keywordAuthor | Deglycosylation | - |
dc.subject.keywordAuthor | Biotransformation | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordPlus | COMPOUND K SUPPRESSES | - |
dc.subject.keywordPlus | CORYNEBACTERIUM-GLUTAMICUM | - |
dc.subject.keywordPlus | PANAX-GINSENG | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | F1 | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | METABOLITE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | KERATINOCYTES | - |
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