Aptamer-functionalized plasmonic Au nanostructures for selective multiplexing of trace-amount molecules via SERS = 플라즈모닉 금 나노구조체의 압타머 기능화를 통한 미량 물질의 선택적 다중 검출 SERS

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Multiplexing of molecules with high sensitivity and selectivity is an essential technology in various fields. Detection of multiple species can significantly reduce analytical time and help provide thorough information from a single sample in applications such as diagnostics, pollution tests, and food safety. So far, various sensor technologies based on electrochemical response and optical signals have been developed to achieve multiplex detection. However, single electrochemical sensing components lack selectivity and thus fabrication of sensor arrays or data processing such as principle component analysis (PCA) is an inevitable step. Recently, reporter-based surface-enhanced Raman spectroscopy (SERS) has drawn much attention as a sensing technology for multiplex detection of trace-amount molecules. Despite its single-molecule level sensitivity and characteristic spectra of Raman reporter molecules, reporter-based SERS still faces the challenge of false positive response. In this thesis, multiplex detection via direct signal of target molecules is achieved by functionalizing aptamers onto SERS substrates with highly dense hot spots. Single-stranded DNA aptamers were anchored onto 3-dimensional plasmonic nanostructures consisted of well-ordered 20 nm Au nanorods by covalent thiolate bonds. The resulting aptamer-functionalized SERS substrates were able to selectively multiplex 3 different small molecules down to the nM level and also perform quantitative analysis for mixed samples of various compositions with high reproducibility.
Advisors
Jung, Yeon Sikresearcher정연식researcher
Description
한국과학기술원 :신소재공학과,
Publisher
한국과학기술원
Issue Date
2018
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 신소재공학과, 2018.8,[iv, 42 p. :]

Keywords

Multiplexing▼aselective detection▼atrace-amount▼asurface-enhanced Raman spectroscopy▼aSERS▼aAptamer; 다중 검출▼a선택적 검출▼a미량 물질▼a표면 증강 라만 산란▼a압타머

URI
http://hdl.handle.net/10203/266509
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=828540&flag=dissertation
Appears in Collection
MS-Theses_Master(석사논문)
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