DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Chan Beum | - |
dc.contributor.advisor | 박찬범 | - |
dc.contributor.author | Yoon, Jaeho | - |
dc.date.accessioned | 2019-09-03T02:45:40Z | - |
dc.date.available | 2019-09-03T02:45:40Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=843308&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/266452 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 신소재공학과, 2019.2,[iv, 38 p. :] | - |
dc.description.abstract | We report visible light-driven, asymmetric hydrogenation of C=C bonds using an ene-reductase from Thermus scotoductus SA-01 (TsOYE) and a light-harvesting dye (rose bengal, RB) co-immobilized in alginate hydrogel. Highly efficient encapsulation of RB in alginate hydrogel was achieved using the intrinsic affinity between TsOYE and RB, which allowed for the construction of robust RB-TsOYE-loaded alginate capsules. In the absence of NADH, the photobiocatalytic system facilitated asymmetric reduction of 2-methylcyclohexenone to an enantiopure (R)-2-methylcyclohexanone (ee>99%, max. conversion: 70.4%, turnover frequency: 1.54 $min^{-1}$, turnover number: 300.2) under illumination. A series of stability tests revealed a significant enhancement of TsOYE's robustness in alginate hydrogel against heat and chemical denaturants. This study provides insight into a greener and sustainable approach of cofactor-free OYE catalysis for producing value-added chemicals using light energy. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | photobiocatalysis▼aene-reductase▼aasymmetric hydrogenation▼aNADH-free bioprocess▼aalginate hydrogel | - |
dc.subject | 구황색 효소▼a탄소 이중결합 비대칭 환원▼a광생체촉매반응▼a니코틴아마이드 보조인자 부재 생체촉매반응▼a알긴산 하이드로젤 | - |
dc.title | Light-harvesting alginate hydrogel for sustainable photobiocatalysis | - |
dc.title.alternative | 광수확 알긴산 하이드로젤을 이용한 지속 가능한 생체촉매 기반 광합성 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :신소재공학과, | - |
dc.contributor.alternativeauthor | 윤재호 | - |
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