Metabolic engineering of Escherichia coli for the production of phenol and anthranilic acid = 대장균에서의 페놀과 안트라닐산염 생산을 위한 대사공학적 기법개발

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Since the majority of aromatic chemicals are currently manufactured employing petroleum as the raw material, development of alternate sustainable production methods have been a driving force. In this study, Escherichia coli was metabolically engineered for the production of phenol and anthranilic acid. For phenol production, I examined 18 E. coli strains with respect to their precursor production, product tolerance, and enzyme activity in search for the host strain for phenol production by introducing tyrosine phenol-lyase(tpl). Among the strains, engineering E. coli BL21 showed the highest phenol tier in flask culture and produced 1.69 g/L of phenol in fed-batch cultures. To solve the toxicity problem, two-phase extractive fermentation was performed and then produced 5.62 g/L of phenol finally. W3110trpD9923 which revealed a nonsense mutation in the trpD gene, showed the probability to accumulate anthranilic acid by the loss of anthranilic acid phosphoribosyl transferase activity, but maintain anthranilic acid synthase activity. The effects of expressing genes of $aroG^{fbr}$, tktA, ppsA, aroK and aroC were evaluated. In shake flask experiments with minimal medium, strains W3110trpD9923/pAnt-G and W3110trpD9923/pAnt2 displayed the best production parameters, accumulating 1.07 - 1.13 g/L of anthranilic acid.
Advisors
Lee, Sang Yupresearcher이상엽researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2013.8,[v, 49 p. :]

Keywords

phenol▼aanthranilic acid▼ametabolic engineering▼asynthetic sRNA▼atwo-phase extractive fermentation; 페놀▼a안트라닐산염▼a대사공학▼a합성 sRNA▼atwo-phasic 발효

URI
http://hdl.handle.net/10203/266348
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=848987&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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