Environmental problems including climate change are urging us to establish sustainable chemical industry that produces chemicals, fuels and materials from renewable biomass using eco-friendly methods. The C5 platform chemicals including 5-aminovaleric acid (AVA) and glutaric acid can be used to produce valerolactam, 5-hydroxyvalerate and 1,5-pentanediol all in which can be used in turn for polyester, polyamide and polyol manufacture. The industrial strain, C. glutamicum, is widely used for global L-lysine, L-gluatmate and cadaverine market demand. Here, an efficient method to produce glutaric acid production by metabolic engineering of C. glutamicum is presented. The L-lysine biosynthetic pathway was extended by heterologous expression of L-lysine catabolic pathway to produce 100.22 g/L glutaric acid in a laboratory-scale bioreactor.