Photothermally activated protein-producing liposomes using gold nanorods = 금 나노막대의 광열 효과를 이용한 조건적 무세포 단백질 발현 리포좀 기술 개발

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Therapeutic proteins are used for various kinds of treatment. However, treatment of therapeutic proteins has limitation which is un-wanted side effects. To overcome the limitation, controlling expression of proteins is essential. Cell-free protein synthesis (CFPS) is the technology for expressing proteins not inside cells. Various kinds of components for protein expression are prepared and mixed with DNA, for example, amino acids, ions, kinases, and cell extracts. By using this synthesis system, expressing proteins artificially without cell can be possible. However, controlling expression of proteins is hard to achieve because DNA undergoes transcription immediately when they mixed with expression components. RNA thermometer (RNAt), thermo-sensitive riboswitch, is the technology for controlling protein expression. Unless RNAt reaches to proper temperature, they don’t undergo translation process. Here, we use RNAt for controlling protein translation. To activate translation of RNAt, we use photo-thermal effect by using gold nanorods (GNRs) with NIR laser. GNRs were photo-excited by NIR laser, which can penetrate the tissue well, therefore we can achieve translation of RNAt when we stimulate. To deliver and express proteins at target site, we loaded cell-free protein synthesis system into liposome which is safe delivery system for cargos. Finally, we confirmed protein translation of RNAt inside liposome using GNRs by photo-thermal effects with NIR laser.
Advisors
Park, Ji Horesearcher박지호researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2017.2,[ix, 37 p. :]

Keywords

Liposomes▼acell-free protein synthesis▼aRNA thermometer▼aphotothermal effect▼agold nanorods; 리포좀▼a무세포 단백질 발현▼a온도 반응 리보핵산▼a광열 효과▼a금 나노 막대

URI
http://hdl.handle.net/10203/266168
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=867010&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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